Development and validation of a multiplex real-time qPCR assay using GMP-grade reagents for leprosy diagnosis

• Fernanda Saloum de Neves Manta ,

  Contributed equally to this work with: Fernanda Saloum de Neves Manta, Thiago Jacomasso

  Roles Data curation, Formal analysis, Investigation, Methodology, Validation, Writing – original draft, Writing – review & editing

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil

  ⨯
• Thiago Jacomasso ,

  Contributed equally to this work with: Fernanda Saloum de Neves Manta, Thiago Jacomasso

  Roles Data curation, Formal analysis, Investigation, Methodology, Software, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Instituto de Biologia Molecular do Paraná, FIOCRUZ, Curitiba, Brazil

  https://orcid.org/0000-0002-1348-1523

  ⨯
• Rita de Cássia Pontello Rampazzo,

  Roles Investigation, Methodology

  Affiliation Instituto de Biologia Molecular do Paraná, FIOCRUZ, Curitiba, Brazil

  https://orcid.org/0000-0002-8367-999X

  ⨯
• Suelen Justo Maria Moreira,

  Roles Data curation, Investigation

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil

  ⨯
• Najua M. Zahra,

  Roles Investigation, Validation

  Affiliation Instituto de Biologia Molecular do Paraná, FIOCRUZ, Curitiba, Brazil

  https://orcid.org/0000-0001-8086-2441

  ⨯
• Stewart T. Cole,

  Roles Supervision

  Affiliations Global Health Institute, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland, Institut Pasteur, Paris, France

  ⨯
• Charlotte Avanzi,

  Roles Data curation, Formal analysis, Investigation

  Affiliations Global Health Institute, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland, Department of Microbiology, Immunology and Pathology, Mycobacteria Research Laboratories, Colorado State University, Fort Collins, Colorado, United States of America

  ⨯
• Thyago Leal-Calvo,

  Roles Data curation, Formal analysis, Investigation

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil

  ⨯
• Sidra Ezidio Gonçalves Vasconcellos,

  Roles Investigation

  Affiliation Laboratório de Biologia Molecular Aplicada a Micobactérias, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil

  ⨯
• Phillip Suffys,

  Roles Resources, Supervision

  Affiliation Laboratório de Biologia Molecular Aplicada a Micobactérias, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil

  ⨯
• Marcelo Ribeiro-Alves,

  Roles Methodology

  Affiliation Instituto Nacional de Infectologia Evandro Chagas, Rio de Janeiro, Brazil

  https://orcid.org/0000-0002-8663-3364

  ⨯
• Marco Aurelio Krieger,

  Roles Funding acquisition, Resources

  Affiliations Instituto de Biologia Molecular do Paraná, FIOCRUZ, Curitiba, Brazil, Laboratório de Ciências e Tecnologias Aplicadas à Saúde (LaCTAS), Instituto Carlos Chagas, Fundação Oswaldo Cruz/FIOCRUZ, Curitiba, Brazil

  ⨯
• Alexandre Dias Tavares Costa ,

  Roles Conceptualization, Methodology, Project administration, Supervision, Writing – review & editing

  * E-mail: alexandre.costa@fiocruz.br (ADTC); milton.moraes@fiocruz.br (MOM)

  ¶‡ ADTC and MOM also contributed equally to this work.

  Affiliations Instituto de Biologia Molecular do Paraná, FIOCRUZ, Curitiba, Brazil, Laboratório de Ciências e Tecnologias Aplicadas à Saúde (LaCTAS), Instituto Carlos Chagas, Fundação Oswaldo Cruz/FIOCRUZ, Curitiba, Brazil

  https://orcid.org/0000-0001-8434-2152

  ⨯
• Milton Ozório Moraes

  Roles Conceptualization, Funding acquisition, Methodology, Resources, Supervision, Writing – review & editing

  * E-mail: alexandre.costa@fiocruz.br (ADTC); milton.moraes@fiocruz.br (MOM)

  ¶‡ ADTC and MOM also contributed equally to this work.

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil

  ⨯