Evaluation of conventional and four real-time PCR methods for the detection of Leishmania on field-collected samples in Ethiopia

• Behailu Merdekios ,

  Contributed equally to this work with: Behailu Merdekios, Myrthe Pareyn

  Roles Conceptualization, Investigation, Validation, Writing – original draft, Writing – review & editing

  * E-mail: merdekib@tcd.ie

  Affiliation Department of Public Health, Arba Minch University, Arba Minch, Ethiopia

  https://orcid.org/0000-0002-8378-241X

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• Myrthe Pareyn ,

  Contributed equally to this work with: Behailu Merdekios, Myrthe Pareyn

  Roles Conceptualization, Formal analysis, Methodology, Validation, Writing – original draft, Writing – review & editing

  Affiliation Evolutionary Ecology Group, University of Antwerp, Antwerp, Belgium

  https://orcid.org/0000-0002-8918-3901

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• Dagimawie Tadesse,

  Roles Investigation, Validation, Visualization

  Affiliation Department of Public Health, Arba Minch University, Arba Minch, Ethiopia

  https://orcid.org/0000-0002-3770-1957

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• Nigatu Eligo,

  Roles Investigation, Validation

  Affiliation Department of Medical Entomology, Arba Minch University, Arba Minch, Ethiopia

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• Mekibib Kassa,

  Roles Investigation, Validation

  Affiliation Leishmaniasis Research and Treatment Centre, University of Gondar, Gondar, Ethiopia

  https://orcid.org/0000-0002-3612-5716

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• Bart K. M. Jacobs,

  Roles Formal analysis, Software, Visualization

  Affiliation Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium

  https://orcid.org/0000-0002-4677-0911

  ⨯
• Herwig Leirs,

  Roles Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

  Affiliation Evolutionary Ecology Group, University of Antwerp, Antwerp, Belgium

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• Jean-Pierre Van Geertruyden,

  Roles Funding acquisition, Project administration, Resources, Writing – review & editing

  Affiliation Global Health Institute, University of Antwerp, Antwerp, Belgium

  https://orcid.org/0000-0001-5006-6364

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• Johan van Griensven,

  Roles Project administration, Resources, Supervision, Writing – review & editing

  Affiliation Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium

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• Guy Caljon,

  Roles Funding acquisition, Methodology, Resources, Writing – review & editing

  Affiliation Laboratory of Microbiology, Parasitology and Hygiene, University of Antwerp, Antwerp, Belgium

  https://orcid.org/0000-0002-4870-3202

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• Lieselotte Cnops

  Roles Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliation Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium

  https://orcid.org/0000-0003-0658-1291

  ⨯