Duplex real-time PCR for sexing Schistosoma japonicum cercariae based on W chromosome-specific genes and its applications

Shuai Liu; Xianyu Piao; Nan Hou; Pengfei Cai; Yu Ma; Qijun Chen

doi:10.1371/journal.pntd.0008609

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Open Access

Peer-reviewed

Research Article

Duplex real-time PCR for sexing Schistosoma japonicum cercariae based on W chromosome-specific genes and its applications

Shuai Liu ,

Roles Conceptualization, Data curation, Funding acquisition, Methodology, Resources, Supervision, Visualization, Writing – original draft

* E-mail: liushuai@ipbcams.ac.cn (SL); qijunchen759@syau.edu.cn (QC)

Affiliation NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China

http://orcid.org/0000-0003-4678-0607

⨯
Xianyu Piao,

Roles Methodology, Project administration, Visualization

Affiliation NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China
⨯
Nan Hou,

Roles Data curation, Investigation, Software, Validation

Affiliation NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China
⨯
Pengfei Cai,

Roles Formal analysis, Software

Affiliation Molecular Parasitology Laboratory, QIMR Berghofer Medical Research Institute, Brisbane, Australia
⨯
Yu Ma,

Roles Data curation

Affiliation NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China
⨯
Qijun Chen

Roles Conceptualization, Resources, Supervision, Writing – review & editing

* E-mail: liushuai@ipbcams.ac.cn (SL); qijunchen759@syau.edu.cn (QC)

Affiliations NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, P.R. China, Key Laboratory of Livestock Infectious Diseases in Northeast China, Ministry of Education, Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agriculture University, Shenyang, P.R. China
⨯

Duplex real-time PCR for sexing Schistosoma japonicum cercariae based on W chromosome-specific genes and its applications

Shuai Liu,
Xianyu Piao,
Nan Hou,
Pengfei Cai,
Yu Ma,
Qijun Chen

Published: August 21, 2020
https://doi.org/10.1371/journal.pntd.0008609

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Polymerase chain reaction
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Schistosoma japonicum
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Schistosoma
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W chromosomes
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