Ultra-sensitive detection of Mycobacterium leprae: DNA extraction and PCR assays

• Fernanda Saloum de Neves Manta,

  Roles Conceptualization, Investigation, Methodology, Writing – original draft

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz—Fiocruz, Rio de Janeiro, RJ, Brazil

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• Thyago Leal-Calvo,

  Roles Formal analysis, Methodology, Writing – original draft

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz—Fiocruz, Rio de Janeiro, RJ, Brazil

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• Suelen Justo M. Moreira,

  Roles Methodology

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz—Fiocruz, Rio de Janeiro, RJ, Brazil

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• Brunna L. C. Marques,

  Roles Methodology

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz—Fiocruz, Rio de Janeiro, RJ, Brazil

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• Marcelo Ribeiro-Alves,

  Roles Formal analysis, Methodology

  Affiliation Laboratório de Pesquisa Clínica em DST-AIDS, Instituto Nacional de Infectologia Evandro Chagas—Fiocruz, Rio de Janeiro, RJ, Brazil

  http://orcid.org/0000-0002-8663-3364

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• Patrícia S. Rosa,

  Roles Resources

  Affiliation Instituto Lauro de Souza Lima, Bauru, SP, Brazil

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• José Augusto C. Nery,

  Roles Resources

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz—Fiocruz, Rio de Janeiro, RJ, Brazil

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• Rita de Cássia Pontello Rampazzo,

  Roles Methodology, Resources

  Affiliation Instituto de Biologia Molecular do Paraná—Fiocruz, Curitiba, PR, Brazil

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• Alexandre Dias Tavares Costa,

  Roles Resources, Supervision

  Affiliations Instituto de Biologia Molecular do Paraná—Fiocruz, Curitiba, PR, Brazil, Instituto Carlos Chagas, Fundação Oswaldo Cruz—Fiocruz, Curitiba, PR, Brazil

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• Marco Aurelio Krieger,

  Roles Funding acquisition, Project administration, Resources

  Affiliations Instituto de Biologia Molecular do Paraná—Fiocruz, Curitiba, PR, Brazil, Instituto Carlos Chagas, Fundação Oswaldo Cruz—Fiocruz, Curitiba, PR, Brazil

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• Milton Ozório Moraes

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – original draft

  * E-mail: milton.moraes@fiocruz.br

  Affiliation Laboratório de Hanseníase, Instituto Oswaldo Cruz—Fiocruz, Rio de Janeiro, RJ, Brazil

  http://orcid.org/0000-0003-2653-0037

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