Nested PCR and the TaqMan SNP Genotyping Assay enhanced the sensitivity of drug resistance testing of Mycobacterium leprae using clinical specimens of leprosy patients

Xiaohua Chen; Jun He; Jian Liu; Yuangang You; Lianchao Yuan; Yan Wen

doi:10.1371/journal.pntd.0007946

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Open Access

Peer-reviewed

Research Article

Nested PCR and the TaqMan SNP Genotyping Assay enhanced the sensitivity of drug resistance testing of Mycobacterium leprae using clinical specimens of leprosy patients

Xiaohua Chen ,

Roles Formal analysis, Investigation, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

* E-mail: hannahchen2003@163.com (XC); weny8@163.com (YW)

Affiliations Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China, Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Diseases, Capital Medical University, Beijing, China
⨯
Jun He,

Roles Data curation, Resources

Affiliation The Center for Disease Control and Prevention of Yunnan Province, Kunming, China
⨯
Jian Liu,

Roles Data curation, Resources

Affiliations Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China, Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Diseases, Capital Medical University, Beijing, China
⨯
Yuangang You,

Roles Data curation, Methodology

Affiliations Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China, Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Diseases, Capital Medical University, Beijing, China
⨯
Lianchao Yuan,

Roles Data curation, Formal analysis

Affiliations Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China, Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Diseases, Capital Medical University, Beijing, China
⨯
Yan Wen

Roles Data curation, Funding acquisition, Investigation, Methodology, Project administration, Resources

* E-mail: hannahchen2003@163.com (XC); weny8@163.com (YW)

Affiliations Beijing Tropical Medicine Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China, Beijing Key Laboratory for Research on Prevention and Treatment of Tropical Diseases, Capital Medical University, Beijing, China

http://orcid.org/0000-0002-5792-6299

⨯

Nested PCR and the TaqMan SNP Genotyping Assay enhanced the sensitivity of drug resistance testing of Mycobacterium leprae using clinical specimens of leprosy patients

Xiaohua Chen,
Jun He,
Jian Liu,
Yuangang You,
Lianchao Yuan,
Yan Wen

Published: December 27, 2019
https://doi.org/10.1371/journal.pntd.0007946

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Leprosy
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Mycobacterium leprae
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Polymerase chain reaction
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Single nucleotide polymorphisms
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Nested polymerase chain reaction
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Genotyping
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Homozygosity
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Variant genotypes
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