Development of RT-qPCR and semi-nested RT-PCR assays for molecular diagnosis of hantavirus pulmonary syndrome

• Bruno Tardelli Diniz Nunes,

  Roles Data curation, Formal analysis, Methodology, Validation, Visualization, Writing – original draft, Writing – review & editing

  Affiliations Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil, Post-Graduation Program in Virology, Evandro Chagas Institute, Ananindeua, Brazil

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• Maria Helena Rodrigues de Mendonça,

  Roles Methodology, Writing – review & editing

  Affiliation Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil

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• Darlene de Brito Simith,

  Roles Data curation, Investigation, Methodology, Writing – review & editing

  Affiliations Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil, Post-Graduation Program in Virology, Evandro Chagas Institute, Ananindeua, Brazil

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• Adriana Freitas Moraes,

  Roles Methodology, Writing – review & editing

  Affiliation Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil

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• Carla Conceição Cardoso,

  Roles Methodology, Writing – review & editing

  Affiliation Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil

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• Ivy Tsuya Essashika Prazeres,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil

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• Ana Alice de Aquino,

  Roles Methodology, Writing – review & editing

  Affiliation Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil

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• Alessandra da Conceição Miranda Santos,

  Roles Methodology, Writing – review & editing

  Affiliation Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil

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• Alice Louize Nunes Queiroz,

  Roles Methodology, Writing – review & editing

  Affiliation Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil

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• Daniela Sueli Guerreiro Rodrigues,

  Roles Methodology, Writing – review & editing

  Affiliation Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil

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• Regis Bruni Andriolo,

  Roles Data curation, Validation, Visualization, Writing – review & editing

  Affiliation Department of Community Health, Pará Estate University, Belém, Brazil

  http://orcid.org/0000-0003-0306-5750

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• Elizabeth Salbé Travassos da Rosa,

  Roles Supervision, Visualization, Writing – review & editing

  Affiliation Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil

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• Livia Carício Martins,

  Roles Supervision, Visualization, Writing – review & editing

  Affiliation Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil

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• Pedro Fernando da Costa Vasconcelos,

  Roles Conceptualization, Formal analysis, Funding acquisition, Project administration, Resources, Supervision, Writing – review & editing

  Affiliations Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil, Post-Graduation Program in Virology, Evandro Chagas Institute, Ananindeua, Brazil

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• Daniele Barbosa de Almeida Medeiros

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Writing – review & editing

  * E-mail: danielemedeiros@iec.pa.gov.br

  Affiliations Department of Arbovirology and Hemorrhagic Fevers, Evandro Chagas Institute, Ananindeua, Brazil, Post-Graduation Program in Virology, Evandro Chagas Institute, Ananindeua, Brazil

  http://orcid.org/0000-0002-3939-0611

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