Utilization of proliferable extracellular amastigotes for transient gene expression, drug sensitivity assay, and CRISPR/Cas9-mediated gene knockout in Trypanosoma cruzi

• Yuko Takagi ,

  Contributed equally to this work with: Yuko Takagi, Yukie Akutsu

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Methodology, Writing – original draft

  * E-mail: yuko-takagi@aist.go.jp

  Affiliation Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan

  http://orcid.org/0000-0002-4567-0986

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• Yukie Akutsu ,

  Contributed equally to this work with: Yuko Takagi, Yukie Akutsu

  Roles Conceptualization, Data curation, Formal analysis, Methodology, Writing – original draft

  Affiliation Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan

  http://orcid.org/0000-0002-6638-070X

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• Motomichi Doi,

  Roles Conceptualization, Writing – original draft

  Affiliation Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan

  http://orcid.org/0000-0002-7837-3056

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• Koji Furukawa

  Roles Conceptualization, Funding acquisition

  Affiliation Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Tsukuba, Ibaraki, Japan

  http://orcid.org/0000-0002-5701-2497

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