Expanding the toolbox for Trypanosoma cruzi: A parasite line incorporating a bioluminescence-fluorescence dual reporter and streamlined CRISPR/Cas9 functionality for rapid in vivo localisation and phenotyping

• Fernanda Cristina Costa,

  Roles Conceptualization, Data curation, Funding acquisition, Investigation, Methodology, Validation, Writing – review & editing

  Affiliations Faculty of Infectious and Tropical diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom, Institute of Physics of São Carlos, University of São Paulo, São Carlos, Brazil

  http://orcid.org/0000-0001-7108-3278

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• Amanda Fortes Francisco,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation Faculty of Infectious and Tropical diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

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• Shiromani Jayawardhana,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation Faculty of Infectious and Tropical diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

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• Simone Guedes Calderano,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliations Faculty of Infectious and Tropical diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom, Laboratory of Parasitology, Instituto Butantan, São Paulo, Brazil

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• Michael D. Lewis,

  Roles Methodology, Resources, Writing – review & editing

  Affiliation Faculty of Infectious and Tropical diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

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• Francisco Olmo,

  Roles Investigation, Methodology, Writing – review & editing

  Affiliation Faculty of Infectious and Tropical diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

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• Tom Beneke,

  Roles Methodology, Resources, Writing – review & editing

  Affiliation Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom

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• Eva Gluenz,

  Roles Methodology, Resources, Writing – review & editing

  Affiliation Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom

  http://orcid.org/0000-0003-4346-8896

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• Jack Sunter,

  Roles Methodology, Resources, Writing – review & editing

  Affiliation Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, United Kingdom

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• Samuel Dean,

  Roles Methodology, Resources, Writing – review & editing

  Affiliation Sir William Dunn School of Pathology, University of Oxford, Oxford, United Kingdom

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• John Morrison Kelly ,

  Roles Conceptualization, Funding acquisition, Project administration, Supervision, Writing – review & editing

  * E-mail: john.kelly@lshtm.ac.uk (JMK); martin.taylor@lshtm.ac.uk (MCT)

  Affiliation Faculty of Infectious and Tropical diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

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• Martin Craig Taylor

  Roles Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Project administration, Resources, Supervision, Validation, Visualization, Writing – original draft, Writing – review & editing

  * E-mail: john.kelly@lshtm.ac.uk (JMK); martin.taylor@lshtm.ac.uk (MCT)

  Affiliation Faculty of Infectious and Tropical diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom

  http://orcid.org/0000-0003-4147-0693

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