First-time detection and identification of the Mycobacterium tuberculosis Complex members in extrapulmonary tuberculosis clinical samples in south Tunisia by a single tube tetraplex real-time PCR assay

Mariam Siala; Salma Smaoui; Wafa Taktak; Salma Hachicha; Asma Ghorbel; Chema Marouane; Sana Kammoun; Dhikrayet Gamara; Leila Slim; Radhouane Gdoura; Férièle Messadi-Akrout

doi:10.1371/journal.pntd.0005572

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Open Access

Peer-reviewed

Research Article

First-time detection and identification of the Mycobacterium tuberculosis Complex members in extrapulmonary tuberculosis clinical samples in south Tunisia by a single tube tetraplex real-time PCR assay

Mariam Siala ,

* E-mail: mariamsiela@gmail.com

Affiliations Department of Biology, Preparatory Institute for Engineering Studies, Sfax, University of Sfax-Tunisia, Department of Life Sciences, Research Laboratory of Environmental Toxicology-Microbiology and Health (LR17ES06), Faculty of Sciences, Sfax, University of Sfax-Tunisia
⨯
Salma Smaoui,

Affiliations Department of Microbiology, Regional hygiene care mycobacteriology laboratory, Hedi-Chaker University Hospital, Sfax, Tunisia, Department of Biology B, Faculty of pharmacy, Monastir, University of Monastir-Tunisia, Department of Microbiology, National Reference Laboratory of Mycobacteria, Research Unit (UR12SP18), A. Mami University Hospital of Pneumology, Ariana, Tunisia
⨯
Wafa Taktak,

Affiliation Department of Life Sciences, Research Laboratory of Environmental Toxicology-Microbiology and Health (LR17ES06), Faculty of Sciences, Sfax, University of Sfax-Tunisia
⨯
Salma Hachicha,

Affiliations Department of Microbiology, Regional hygiene care mycobacteriology laboratory, Hedi-Chaker University Hospital, Sfax, Tunisia, Department of Biology B, Faculty of pharmacy, Monastir, University of Monastir-Tunisia
⨯
Asma Ghorbel,

Affiliations Department of Microbiology, Regional hygiene care mycobacteriology laboratory, Hedi-Chaker University Hospital, Sfax, Tunisia, Department of Biology B, Faculty of pharmacy, Monastir, University of Monastir-Tunisia
⨯
Chema Marouane,

Affiliations Department of Microbiology, Regional hygiene care mycobacteriology laboratory, Hedi-Chaker University Hospital, Sfax, Tunisia, Department of Biology B, Faculty of pharmacy, Monastir, University of Monastir-Tunisia, Department of Microbiology, National Reference Laboratory of Mycobacteria, Research Unit (UR12SP18), A. Mami University Hospital of Pneumology, Ariana, Tunisia
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Sana Kammoun,

Affiliations Department of Microbiology, Regional hygiene care mycobacteriology laboratory, Hedi-Chaker University Hospital, Sfax, Tunisia, Department of Biology B, Faculty of pharmacy, Monastir, University of Monastir-Tunisia, Department of Microbiology, National Reference Laboratory of Mycobacteria, Research Unit (UR12SP18), A. Mami University Hospital of Pneumology, Ariana, Tunisia
⨯
Dhikrayet Gamara,

Affiliation Care management and basic health, Ministry of Health, Tunis, Tunisia
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Leila Slim,

Affiliations Department of Biology B, Faculty of pharmacy, Monastir, University of Monastir-Tunisia, Department of Microbiology, National Reference Laboratory of Mycobacteria, Research Unit (UR12SP18), A. Mami University Hospital of Pneumology, Ariana, Tunisia
⨯
Radhouane Gdoura,

Affiliation Department of Life Sciences, Research Laboratory of Environmental Toxicology-Microbiology and Health (LR17ES06), Faculty of Sciences, Sfax, University of Sfax-Tunisia
⨯
Férièle Messadi-Akrout

Affiliations Department of Microbiology, Regional hygiene care mycobacteriology laboratory, Hedi-Chaker University Hospital, Sfax, Tunisia, Department of Biology B, Faculty of pharmacy, Monastir, University of Monastir-Tunisia, Department of Microbiology, National Reference Laboratory of Mycobacteria, Research Unit (UR12SP18), A. Mami University Hospital of Pneumology, Ariana, Tunisia
⨯

First-time detection and identification of the Mycobacterium tuberculosis Complex members in extrapulmonary tuberculosis clinical samples in south Tunisia by a single tube tetraplex real-time PCR assay

Mariam Siala,
Salma Smaoui,
Wafa Taktak,
Salma Hachicha,
Asma Ghorbel,
Chema Marouane,
Sana Kammoun,
Dhikrayet Gamara,
Leila Slim,
Radhouane Gdoura

Published: May 5, 2017
https://doi.org/10.1371/journal.pntd.0005572

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