qPCR-High resolution melt analysis for drug susceptibility testing of Mycobacterium leprae directly from clinical specimens of leprosy patients

• Sergio Araujo ,

  * E-mail: saraujo@gmx.com (SA); dwilliams2@hrsa.gov, DNARNA21@aol.com (DLW)

  Affiliations National Reference Center for Sanitary Dermatology and Leprosy, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil, Post-Graduate Program in Health Sciences, School of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil, Division National Hansen's Disease Programs (NHDP), Healthcare Systems Bureau (HSB), Health Resources and Services Administration (HRSA), U.S. Department of Health and Human Services (DHHS), Baton Rouge, Louisiana, United States of America

  http://orcid.org/0000-0001-8666-4948

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• Luiz Ricardo Goulart,

  Affiliations National Reference Center for Sanitary Dermatology and Leprosy, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil, Post-Graduate Program in Health Sciences, School of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil, Institute of Genetics and Biochemistry, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil

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• Richard W. Truman,

  Affiliation Division National Hansen's Disease Programs (NHDP), Healthcare Systems Bureau (HSB), Health Resources and Services Administration (HRSA), U.S. Department of Health and Human Services (DHHS), Baton Rouge, Louisiana, United States of America

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• Isabela Maria B. Goulart,

  Affiliations National Reference Center for Sanitary Dermatology and Leprosy, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil, Post-Graduate Program in Health Sciences, School of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil

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• Varalakshmi Vissa,

  Affiliation Good Samaritan Society, Fort Collins, Colorado, United States of America

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• Wei Li,

  Affiliation Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America

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• Masanori Matsuoka,

  Affiliation Leprosy Research Center, National Institute of Infectious Diseases, Tokyo, Japan

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• Philip Suffys,

  Affiliations Fundação Oswaldo Cruz, Laboratory of Molecular Biology applied to Mycobacteria, Rio de Janeiro, Rio de Janeiro, Brazil, Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium

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• Amanda B. Fontes,

  Affiliation Fundação Oswaldo Cruz, Laboratory of Molecular Biology applied to Mycobacteria, Rio de Janeiro, Rio de Janeiro, Brazil

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• Patricia S. Rosa,

  Affiliation Instituto Lauro de Souza Lima, Department of Biology, Bauru, São Paulo, Brazil

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• David M. Scollard,

  Affiliation Division National Hansen's Disease Programs (NHDP), Healthcare Systems Bureau (HSB), Health Resources and Services Administration (HRSA), U.S. Department of Health and Human Services (DHHS), Baton Rouge, Louisiana, United States of America

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• Diana L. Williams

  * E-mail: saraujo@gmx.com (SA); dwilliams2@hrsa.gov, DNARNA21@aol.com (DLW)

  Affiliation Division National Hansen's Disease Programs (NHDP), Healthcare Systems Bureau (HSB), Health Resources and Services Administration (HRSA), U.S. Department of Health and Human Services (DHHS), Baton Rouge, Louisiana, United States of America

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