Improved Quantification, Propagation, Purification and Storage of the Obligate Intracellular Human Pathogen Orientia tsutsugamushi

Suparat Giengkam; Alex Blakes; Peemdej Utsahajit; Suwittra Chaemchuen; Sharanjeet Atwal; Stuart D. Blacksell; Daniel H. Paris; Nicholas P. J. Day; Jeanne Salje

doi:10.1371/journal.pntd.0004009

Loading metrics

Open Access

Peer-reviewed

Research Article

Improved Quantification, Propagation, Purification and Storage of the Obligate Intracellular Human Pathogen Orientia tsutsugamushi

Suparat Giengkam,

Affiliation Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
⨯
Alex Blakes,

Affiliation Department of Medicine, University of Oxford, Oxford, United Kingdom
⨯
Peemdej Utsahajit,

Affiliation Department of Biochemistry, University of Oxford, Oxford, United Kingdom
⨯
Suwittra Chaemchuen,

Affiliation Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
⨯
Sharanjeet Atwal,

Affiliations Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom
⨯
Stuart D. Blacksell,

Affiliations Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom
⨯
Daniel H. Paris,

Affiliations Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom
⨯
Nicholas P. J. Day,

Affiliations Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom
⨯
Jeanne Salje

* E-mail: jeanne.salje@ndm.ox.ac.uk

Affiliations Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand, Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, United Kingdom
⨯

Improved Quantification, Propagation, Purification and Storage of the Obligate Intracellular Human Pathogen Orientia tsutsugamushi

Suparat Giengkam,
Alex Blakes,
Peemdej Utsahajit,
Suwittra Chaemchuen,
Sharanjeet Atwal,
Stuart D. Blacksell,
Daniel H. Paris,
Nicholas P. J. Day,
Jeanne Salje

Published: August 28, 2015
https://doi.org/10.1371/journal.pntd.0004009

Reader Comments (0)

Post a new comment on this article

Subject Areas
?

For more information about PLOS Subject Areas, click here.
We want your feedback. Do these Subject Areas make sense for this article? Click the target next to the incorrect Subject Area and let us know. Thanks for your help!

Host cells
Is the Subject Area "Host cells" applicable to this article?

Thanks for your feedback.
Orienta tsutsugamushi
Is the Subject Area "Orienta tsutsugamushi" applicable to this article?

Thanks for your feedback.
Lysis (medicine)
Is the Subject Area "Lysis (medicine)" applicable to this article?

Thanks for your feedback.
DNA extraction
Is the Subject Area "DNA extraction" applicable to this article?

Thanks for your feedback.
Intracellular pathogens
Is the Subject Area "Intracellular pathogens" applicable to this article?

Thanks for your feedback.
Bacteria
Is the Subject Area "Bacteria" applicable to this article?

Thanks for your feedback.
L929 cells
Is the Subject Area "L929 cells" applicable to this article?

Thanks for your feedback.
Bacterial growth
Is the Subject Area "Bacterial growth" applicable to this article?

Thanks for your feedback.