High Content Analysis of Primary Macrophages Hosting Proliferating Leishmania Amastigotes: Application to Anti-leishmanial Drug Discovery

Nathalie Aulner; Anne Danckaert; Eline Rouault-Hardoin; Julie Desrivot; Olivier Helynck; Pierre-Henri Commere; Hélène Munier-Lehmann; Gerald F. Späth; Spencer L. Shorte; Geneviève Milon; Eric Prina

doi:10.1371/journal.pntd.0002154

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Open Access

Peer-reviewed

Research Article

High Content Analysis of Primary Macrophages Hosting Proliferating Leishmania Amastigotes: Application to Anti-leishmanial Drug Discovery

Nathalie Aulner ,

* E-mail: nathalie.aulner@pasteur.fr (NA); eric.prina@pasteur.fr (EP)

Affiliation Institut Pasteur, Imagopole, Paris, France
⨯
Anne Danckaert,

Affiliation Institut Pasteur, Imagopole, Paris, France
⨯
Eline Rouault-Hardoin,

Affiliation Institut Pasteur, Laboratoire Immunophysiologie et Parasitisme, Département de Parasitologie et Mycologie, Paris, France
⨯
Julie Desrivot,

Current address: Galápagos Sasu, Parc Biocitech, Romainville, France

Affiliation Institut Pasteur, Laboratoire Immunophysiologie et Parasitisme, Département de Parasitologie et Mycologie, Paris, France
⨯
Olivier Helynck,

Affiliation Institut Pasteur, Unité Chimie et Biocatalyse, Département de Biologie Structurale et Chimie, Paris, France
⨯
Pierre-Henri Commere,

Affiliation Institut Pasteur, Imagopole, Paris, France
⨯
Hélène Munier-Lehmann,

Affiliations Institut Pasteur, Unité Chimie et Biocatalyse, Département de Biologie Structurale et Chimie, Paris, France, CNRS, UMR 3523, Paris, France
⨯
Gerald F. Späth,

Affiliations Institut Pasteur, Unité Parasitologie Moléculaire et Signalisation, Département de Parasitologie et Mycologie, Paris, France, CNRS URA 2581, Paris, France
⨯
Spencer L. Shorte,

Affiliation Institut Pasteur, Imagopole, Paris, France
⨯
Geneviève Milon,

Affiliation Institut Pasteur, Laboratoire Immunophysiologie et Parasitisme, Département de Parasitologie et Mycologie, Paris, France
⨯
Eric Prina

* E-mail: nathalie.aulner@pasteur.fr (NA); eric.prina@pasteur.fr (EP)

Affiliations Institut Pasteur, Laboratoire Immunophysiologie et Parasitisme, Département de Parasitologie et Mycologie, Paris, France, Institut Pasteur, Unité Parasitologie Moléculaire et Signalisation, Département de Parasitologie et Mycologie, Paris, France, CNRS URA 2581, Paris, France
⨯

High Content Analysis of Primary Macrophages Hosting Proliferating Leishmania Amastigotes: Application to Anti-leishmanial Drug Discovery

Nathalie Aulner,
Anne Danckaert,
Eline Rouault-Hardoin,
Julie Desrivot,
Olivier Helynck,
Pierre-Henri Commere,
Hélène Munier-Lehmann,
Gerald F. Späth,
Spencer L. Shorte,
Geneviève Milon

Published: April 4, 2013
https://doi.org/10.1371/journal.pntd.0002154

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Macrophages
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Amastigotes
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Leishmania
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Fluorescence imaging
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Host cells
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