Detection of Viable Mycobacterium ulcerans in Clinical Samples by a Novel Combined 16S rRNA Reverse Transcriptase/IS2404 Real-Time qPCR Assay

Marcus Beissner; Dominik Symank; Richard Odame Phillips; Yaw Ampem Amoako; Nana-Yaa Awua-Boateng; Fred Stephen Sarfo; Moritz Jansson; Kristina Lydia Huber; Karl-Heinz Herbinger; Florian Battke; Thomas Löscher; Ohene Adjei; Gisela Bretzel

doi:10.1371/journal.pntd.0001756

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Detection of Viable Mycobacterium ulcerans in Clinical Samples by a Novel Combined 16S rRNA Reverse Transcriptase/IS2404 Real-Time qPCR Assay

Marcus Beissner ,

Contributed equally to this work with: Marcus Beissner, Dominik Symank

* E-mail: beissner@lrz.uni-muenchen.de

Affiliation Department of Infectious Diseases and Tropical Medicine (DITM), University Hospital, Ludwig-Maximilians University, Munich, Germany
⨯
Dominik Symank ,

Contributed equally to this work with: Marcus Beissner, Dominik Symank

Affiliation Department of Infectious Diseases and Tropical Medicine (DITM), University Hospital, Ludwig-Maximilians University, Munich, Germany
⨯
Richard Odame Phillips,

Affiliation Komfo Anokye Teaching Hospital (KATH), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana
⨯
Yaw Ampem Amoako,

Affiliation Komfo Anokye Teaching Hospital (KATH), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana
⨯
Nana-Yaa Awua-Boateng,

Affiliation Kumasi Centre for Collaborative Research (KCCR), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana
⨯
Fred Stephen Sarfo,

Affiliation Komfo Anokye Teaching Hospital (KATH), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana
⨯
Moritz Jansson,

Affiliation Department of Infectious Diseases and Tropical Medicine (DITM), University Hospital, Ludwig-Maximilians University, Munich, Germany
⨯
Kristina Lydia Huber,

Affiliation Department of Infectious Diseases and Tropical Medicine (DITM), University Hospital, Ludwig-Maximilians University, Munich, Germany
⨯
Karl-Heinz Herbinger,

Affiliation Department of Infectious Diseases and Tropical Medicine (DITM), University Hospital, Ludwig-Maximilians University, Munich, Germany
⨯
Florian Battke,

Affiliation Dr. Battke SCIENTIA GmbH, Taufkirchen, Germany
⨯
Thomas Löscher,

Affiliation Department of Infectious Diseases and Tropical Medicine (DITM), University Hospital, Ludwig-Maximilians University, Munich, Germany
⨯
Ohene Adjei,

Affiliation Komfo Anokye Teaching Hospital (KATH), Kwame Nkrumah University of Science and Technology (KNUST), Kumasi, Ghana
⨯
Gisela Bretzel

Affiliation Department of Infectious Diseases and Tropical Medicine (DITM), University Hospital, Ludwig-Maximilians University, Munich, Germany
⨯

Detection of Viable Mycobacterium ulcerans in Clinical Samples by a Novel Combined 16S rRNA Reverse Transcriptase/IS2404 Real-Time qPCR Assay

Marcus Beissner,
Dominik Symank,
Richard Odame Phillips,
Yaw Ampem Amoako,
Nana-Yaa Awua-Boateng,
Fred Stephen Sarfo,
Moritz Jansson,
Kristina Lydia Huber,
Karl-Heinz Herbinger,
Florian Battke

Published: August 28, 2012
https://doi.org/10.1371/journal.pntd.0001756

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