Comparative Production Analysis of Three Phlebovirus Nucleoproteins under Denaturing or Non-Denaturing Conditions for Crystallographic Studies

Open Access

Peer-reviewed

Research Article

Violaine Lantez,

Affiliation Architecture et Fonction des Macromolécules Biologiques, UMR 6098 Centre National de la Recherche Scientifique, Université de la Méditerranée and Université de Provence, Marseille, France
⨯
Karen Dalle,

Affiliation Architecture et Fonction des Macromolécules Biologiques, UMR 6098 Centre National de la Recherche Scientifique, Université de la Méditerranée and Université de Provence, Marseille, France
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Rémi Charrel,

Affiliation Unité des Virus Emergents, UMR 190, Aix-Marseille Université and Institut de Recherche pour le Développement, Marseille, France
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Cécile Baronti,

Affiliation Unité des Virus Emergents, UMR 190, Aix-Marseille Université and Institut de Recherche pour le Développement, Marseille, France
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Bruno Canard,

Affiliation Architecture et Fonction des Macromolécules Biologiques, UMR 6098 Centre National de la Recherche Scientifique, Université de la Méditerranée and Université de Provence, Marseille, France
⨯
Bruno Coutard

* E-mail: bruno.coutard@afmb.univ-mrs.fr

Affiliation Architecture et Fonction des Macromolécules Biologiques, UMR 6098 Centre National de la Recherche Scientifique, Université de la Méditerranée and Université de Provence, Marseille, France
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Rift Valley fever virus
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SDS polyacrylamide gel electrophoresis
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Proteases
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Nucleoproteins
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Recombinant proteins
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Coomassie Blue staining
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Crystallization
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Imidazole
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