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Zygotes

Highly efficient production of transgenic rats with long DNA insertions using piggyBac transposase mRNA and piezo-assisted microinjection

Kohtaro Morita, Shunya Ihashi, [ ... ], Masahide Asano

Improved protocol for the vitrification and warming of rat zygotes by optimizing the warming solution and oocyte donor age

Naomi Nakagata, Satohiro Nakao, [ ... ], Toru Takeo

Induction of autophagy in one-cell stage somatic cell nuclear transfer embryos improves preimplantation embryonic development in goat species

Nasrin Mahvash, Reza Moradi-Hajidavaloo, [ ... ], Mohammad Hossein Nasr-Esfahani

A simple validation and screening method for CRISPR/Cas9-mediated gene editing in mouse embryos to facilitate genetically modified mice production

Dawid Winiarczyk, Hossein Khodadadi, Paweł Leszczyński, Hiroaki Taniguchi

Cumulus cell expansion, nuclear maturation and embryonic development of bovine cumulus-oocyte complexes matured in varying concentrations of follicular fluid

Verónica M. Negrón-Pérez, Abdullah Al Naib, [ ... ], Michelle L. Rhoads

Two species of the green algae Volvox sect. Volvox from the Japanese ancient lake, Lake Biwa

Hisayoshi Nozaki, Ryo Matsuzaki, [ ... ], Masanobu Kawachi

Zygote cryobanking applied to CRISPR/Cas9 microinjection in mice

Geraldine Schlapp, María Noel Meikle, [ ... ], Martina Crispo

Blastulation time measured with time-lapse system can predict in vitro viability of bovine blastocysts

Carmen Huayhua, Misael Rodríguez, [ ... ], Edwin Mellisho

Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system

Giuliana de Avila Ferronato, Carolina Mônica dos Santos, [ ... ], Juliano Coelho da Silveira

Vitrification of porcine immature oocytes and zygotes results in different levels of DNA damage which reflects developmental competence to the blastocyst stage

Tamás Somfai, Seiki Haraguchi, [ ... ], Kazuhiro Kikuchi

GM-CSF perturbs cell identity in mouse pre-implantation embryos

Tim Pock, Katharina Schulte, [ ... ], Verena Nordhoff

Optimised CO₂-containing medium for in vitro culture and transportation of mouse preimplantation embryos without CO₂ incubator

Yasuyuki Kikuchi, Sayaka Wakayama, [ ... ], Teruhiko Wakayama

Improved cryopreservation of in vitro produced bovine embryos using FGF2, LIF, and IGF1

Katy S. Stoecklein, M. Sofia Ortega, [ ... ], Randall S. Prather

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