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Vector construction

Automated Solid-Phase Subcloning Based on Beads Brought into Proximity by Magnetic Force

Elton P. Hudson, Andrej Nikoshkov, Mathias Uhlen, Johan Rockberg

published 18 May 2012

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Functionally Orthologous Viral and Cellular MicroRNAs Studied by a Novel Dual-Fluorescent Reporter System

Xiangyu You, Zhiping Zhang, Jinyu Fan, Zongqiang Cui, Xian-En Zhang

published 27 Apr 2012

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In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization

Xinglin Jiang, Jianming Yang, Haibo Zhang, Huibin Zou, Cong Wang, Mo Xian

published 26 Jan 2012

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High-Throughput Functional MicroRNAs Profiling by Recombinant AAV-Based MicroRNA Sensor Arrays

Wenhong Tian, Xiaoyan Dong, Xuerong Liu, Gang Wang, Zheyue Dong, Wei Shen, Gang Zheng, Jianxin Lu, Jinzhong Chen, Yue Wang, Zhijian Wu, Xiaobing Wu

published 05 Jan 2012

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Development of Shuttle Vectors for Transformation of Diverse Rickettsia Species

Nicole Y. Burkhardt, Gerald D. Baldridge, Phillip C. Williamson, Peggy M. Billingsley, Chan C. Heu, Roderick F. Felsheim, Timothy J. Kurtti, Ulrike G. Munderloh

published 21 Dec 2011

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Rev-Free HIV-1 Gene Delivery System for Targeting Rev-RRE-Crm1 Nucleocytoplasmic RNA Transport Pathway

Narasimhachar Srinivasakumar

published 02 Dec 2011

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Relative Quantification of Protein-Protein Interactions Using a Dual Luciferase Reporter Pull-Down Assay System

Shuaizheng Jia, Jianchun Peng, Bo Gao, Zhongbin Chen, Yong Zhou, Qiuxia Fu, Haiping Wang, Linsheng Zhan

published 19 Oct 2011

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Broad-Spectrum Antiviral Activity of RNA Interference against Four Genotypes of Japanese Encephalitis Virus Based on Single MicroRNA Polycistrons

Zhiqiang Wu, Ying Xue, Bei Wang, Jiang Du, Qi Jin

published 18 Oct 2011

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Integration-Free iPS Cells Engineered Using Human Artificial Chromosome Vectors

Masaharu Hiratsuka, Narumi Uno, Kana Ueda, Hajime Kurosaki, Natsuko Imaoka, Kanako Kazuki, Etsuya Ueno, Yutaro Akakura, Motonobu Katoh, Mitsuhiko Osaki, Yasuhiro Kazuki, Masato Nakagawa, Shinya Yamanaka, Mitsuo Oshimura

published 05 Oct 2011

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Assembly of Designer TAL Effectors by Golden Gate Cloning

Ernst Weber, Ramona Gruetzner, Stefan Werner, Carola Engler, Sylvestre Marillonnet

published 19 May 2011

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RMDAP: A Versatile, Ready-To-Use Toolbox for Multigene Genetic Transformation

Lei Ma, Jiangli Dong, Yongsheng Jin, Mingliang Chen, Xiaoye Shen, Tao Wang

published 13 May 2011

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Structure of HsdS Subunit from Thermoanaerobacter tengcongensis Sheds Lights on Mechanism of Dynamic Opening and Closing of Type I Methyltransferase

Pu Gao, Qun Tang, XiaoMin An, XiaoXue Yan, DongCai Liang

published 02 Mar 2011

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Zebrafish ProVEGF-C Expression, Proteolytic Processing and Inhibitory Effect of Unprocessed ProVEGF-C during Fin Regeneration

Abdel-Majid Khatib, Rachid Lahlil, Nathalie Scamuffa, Marie-Andrée Akimenko, Sylvain Ernest, Abdderahim Lomri, Claude Lalou, Nabil G. Seidah, Bruno O. Villoutreix, Fabien Calvo, Geraldine Siegfried

published 02 Jul 2010

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