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Vector construction

Construction of New Ligation-Independent Cloning Vectors for the Expression and Purification of Recombinant Proteins in Silkworms Using BmNPV Bacmid System

Tatsuya Kato, James R. Thompson, Enoch Y. Park

An Efficient Procedure for Marker-Free Mutagenesis of S. coelicolor by Site-Specific Recombination for Secondary Metabolite Overproduction

Bo Zhang, Lin Zhang, [ ... ], Xiaoming Ding

The Estrogen-Related Receptor Alpha Upregulates Secretin Expressions in Response to Hypertonicity and Angiotensin II Stimulation

Vien H. Y. Lee, Ian P. Y. Lam, [ ... ], Leo T. O. Lee

Enhanced snoMEN Vectors Facilitate Establishment of GFP–HIF-1α Protein Replacement Human Cell Lines

Motoharu Ono, Kayo Yamada, [ ... ], Angus I. Lamond

Role of Intron-Mediated Enhancement on Accumulation of an Arabidopsis NB-LRR Class R-protein that Confers Resistance to Cucumber mosaic virus

Yukiyo Sato, Sugihiro Ando, Hideki Takahashi

Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene

Aya Bridgeland, Sudip Biswas, [ ... ], Endang M. Septiningsih

A versatile toolbox for knock-in gene targeting based on the Multisite Gateway technology

Sho Yoshimatsu, Takefumi Sone, [ ... ], Hideyuki Okano

Broad-Spectrum Antiviral Activity of RNA Interference against Four Genotypes of Japanese Encephalitis Virus Based on Single MicroRNA Polycistrons

Zhiqiang Wu, Ying Xue, [ ... ], Qi Jin

Functionally Orthologous Viral and Cellular MicroRNAs Studied by a Novel Dual-Fluorescent Reporter System

Xiangyu You, Zhiping Zhang, [ ... ], Xian-En Zhang

Sequences within Both the 5′ UTR and Gag Are Required for Optimal In Vivo Packaging and Propagation of Mouse Mammary Tumor Virus (MMTV) Genomic RNA

Farah Mustafa, Dhuha Al Amri, [ ... ], Tahir A. Rizvi

A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences

Korbinian N. Kropp, Tim J. Schäufele, [ ... ], Catherine Wölfel

A Novel and Simple Method for Rapid Generation of Recombinant Porcine Adenoviral Vectors for Transgene Expression

Peng Zhang, Enqi Du, [ ... ], Zengqi Yang

A Simplified and Versatile System for the Simultaneous Expression of Multiple siRNAs in Mammalian Cells Using Gibson DNA Assembly

Fang Deng, Xiang Chen, [ ... ], Tong-Chuan He

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