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Vector construction

Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen

Yuanping Zhou, Junjie Wang, Ivan Zhou, Haibo Lou, Chang-Zheng Li, Zhen-Rui Chen, Zhe-Huan Zhang, Shuwen Liu, Shuguang Wu, Wanlong Tan, Shibo Jiang, Chen Zhou

published 11 Nov 2013

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Golden GATEway Cloning – A Combinatorial Approach to Generate Fusion and Recombination Constructs

Stephan Kirchmaier, Katharina Lust, Joachim Wittbrodt

published 07 Oct 2013

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Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site

Song Yang, Ye Kuang, Hongbo Li, Yuehong Liu, Xiaoyan Hui, Peng Li, Zhiwu Jiang, Yulai Zhou, Yu Wang, Aimin Xu, Shiwu Li, Pentao Liu, Donghai Wu

published 19 Sep 2013

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Heterologous Reconstitution of the Intact Geodin Gene Cluster in Aspergillus nidulans through a Simple and Versatile PCR Based Approach

Morten Thrane Nielsen, Jakob Blæsbjerg Nielsen, Dianna Chinyere Anyaogu, Dorte Koefoed Holm, Kristian Fog Nielsen, Thomas Ostenfeld Larsen, Uffe Hasbro Mortensen

published 23 Aug 2013

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Gateway Vectors for Efficient Artificial Gene Assembly In Vitro and Expression in Yeast Saccharomyces cerevisiae

Claudiu V. Giuraniuc, Murray MacPherson, Yasushi Saka

published 10 May 2013

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Construction of New Ligation-Independent Cloning Vectors for the Expression and Purification of Recombinant Proteins in Silkworms Using BmNPV Bacmid System

Tatsuya Kato, James R. Thompson, Enoch Y. Park

published 10 May 2013

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Construction of a Single Lentiviral Vector Containing Tetracycline-Inducible Alb-uPA for Transduction of uPA Expression in Murine Hepatocytes

Jiasi Bai, Jungang Li, Qing Mao

published 23 Apr 2013

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A Series of TA-Based and Zero-Background Vectors for Plant Functional Genomics

Chuntao Wang, Xianlun Yin, Xiangxiang Kong, Wansha Li, Lan Ma, Xudong Sun, Yanlong Guan, Christopher D. Todd, Yongping Yang, Xiangyang Hu

published 29 Mar 2013

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Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning

Wataru Fujii, Kiyoshi Kano, Koji Sugiura, Kunihiko Naito

published 25 Mar 2013

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CD24 Induces Expression of the Oncomir miR-21 via Src, and CD24 and Src Are Both Post-Transcriptionally Downregulated by the Tumor Suppressor miR-34a

Santoshi Muppala, Giridhar Mudduluru, Jörg H. Leupold, Daniel Buergy, Jonathan P. Sleeman, Heike Allgayer

published 22 Mar 2013

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Enhancement of the Gene Targeting Efficiency of Non-Conventional Yeasts by Increasing Genetic Redundancy

Zao Chen, Hongbing Sun, Pengfei Li, Ning He, Taicheng Zhu, Yin Li

published 07 Mar 2013

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Parallel In Vivo DNA Assembly by Recombination: Experimental Demonstration and Theoretical Approaches

Zhenyu Shi, Anthony G. Wedd, Sally L. Gras

published 28 Feb 2013

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Exon-Skipping Strategy by Ratio Modulation between Cytoprotective versus Pro-Apoptotic Clusterin Forms Increased Sensitivity of LNCaP to Cell Death

Abdellatif Essabbani, Luis Garcia, Maria Josè Zonetti, Tommaso Fisco, Sabina Pucci, Gilles Chiocchia

published 13 Feb 2013

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