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Vector construction

Simultaneous Expression of Displayed and Secreted Antibodies for Antibody Screen

Yuanping Zhou, Junjie Wang, [ ... ], Chen Zhou

Golden GATEway Cloning – A Combinatorial Approach to Generate Fusion and Recombination Constructs

Stephan Kirchmaier, Katharina Lust, Joachim Wittbrodt

Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site

Song Yang, Ye Kuang, [ ... ], Donghai Wu

Heterologous Reconstitution of the Intact Geodin Gene Cluster in Aspergillus nidulans through a Simple and Versatile PCR Based Approach

Morten Thrane Nielsen, Jakob Blæsbjerg Nielsen, [ ... ], Uffe Hasbro Mortensen

Gateway Vectors for Efficient Artificial Gene Assembly In Vitro and Expression in Yeast Saccharomyces cerevisiae

Claudiu V. Giuraniuc, Murray MacPherson, Yasushi Saka

Construction of New Ligation-Independent Cloning Vectors for the Expression and Purification of Recombinant Proteins in Silkworms Using BmNPV Bacmid System

Tatsuya Kato, James R. Thompson, Enoch Y. Park

Construction of a Single Lentiviral Vector Containing Tetracycline-Inducible Alb-uPA for Transduction of uPA Expression in Murine Hepatocytes

Jiasi Bai, Jungang Li, Qing Mao

A Series of TA-Based and Zero-Background Vectors for Plant Functional Genomics

Chuntao Wang, Xianlun Yin, [ ... ], Xiangyang Hu

Repeatable Construction Method for Engineered Zinc Finger Nuclease Based on Overlap Extension PCR and TA-Cloning

Wataru Fujii, Kiyoshi Kano, Koji Sugiura, Kunihiko Naito

CD24 Induces Expression of the Oncomir miR-21 via Src, and CD24 and Src Are Both Post-Transcriptionally Downregulated by the Tumor Suppressor miR-34a

Santoshi Muppala, Giridhar Mudduluru, [ ... ], Heike Allgayer

Enhancement of the Gene Targeting Efficiency of Non-Conventional Yeasts by Increasing Genetic Redundancy

Zao Chen, Hongbing Sun, [ ... ], Yin Li

Parallel In Vivo DNA Assembly by Recombination: Experimental Demonstration and Theoretical Approaches

Zhenyu Shi, Anthony G. Wedd, Sally L. Gras

Exon-Skipping Strategy by Ratio Modulation between Cytoprotective versus Pro-Apoptotic Clusterin Forms Increased Sensitivity of LNCaP to Cell Death

Abdellatif Essabbani, Luis Garcia, [ ... ], Gilles Chiocchia

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