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Vector construction

Transposition-Based Method for the Rapid Generation of Gene-Targeting Vectors to Produce Cre/Flp-Modifiable Conditional Knock-Out Mice

Hilkka Turakainen, Jonna Saarimäki-Vire, [ ... ], Harri Savilahti

CD24 Induces Expression of the Oncomir miR-21 via Src, and CD24 and Src Are Both Post-Transcriptionally Downregulated by the Tumor Suppressor miR-34a

Santoshi Muppala, Giridhar Mudduluru, [ ... ], Heike Allgayer

Quantitative Effect of Suboptimal Codon Usage on Translational Efficiency of mRNA Encoding HIV-1 gag in Intact T Cells

Kholiswa C. Ngumbela, Kieran P. Ryan, [ ... ], Daniel G. Kavanagh

FabV/Triclosan Is an Antibiotic-Free and Cost-Effective Selection System for Efficient Maintenance of High and Medium -Copy Number Plasmids in Escherichia coli

Syed A. Ali, Yik Wei Chew

Enhancement of the Gene Targeting Efficiency of Non-Conventional Yeasts by Increasing Genetic Redundancy

Zao Chen, Hongbing Sun, [ ... ], Yin Li

A Natural Human Retrovirus Efficiently Complements Vectors Based on Murine Leukemia Virus

Beihua Dong, Robert H. Silverman, Eugene S. Kandel

Tools for Gene-Regulatory Analyses in the Marine Annelid Platynereis dumerilii

Benjamin Backfisch, Vitaly V. Kozin, [ ... ], Florian Raible

Plant X-tender: An extension of the AssemblX system for the assembly and expression of multigene constructs in plants

Tjaša Lukan, Fabian Machens, [ ... ], Kristina Gruden

Novel gateway binary vectors for rapid tripartite DNA assembly and promoter analysis with various reporters and tags in the liverwort Marchantia polymorpha

Shoji Mano, Ryuichi Nishihama, [ ... ], Tsuyoshi Nakagawa

A PCR-Based Method to Construct Lentiviral Vector Expressing Double Tough Decoy for miRNA Inhibition

Huiling Qiu, Jiasheng Zhong, [ ... ], Deming Gou

Species-Specific Chromosome Engineering Greatly Improves Fully Human Polyclonal Antibody Production Profile in Cattle

Hiroaki Matsushita, Akiko Sano, [ ... ], Yoshimi Kuroiwa

A Rapid Cloning Method Employing Orthogonal End Protection

Arjen J. Jakobi, Eric G. Huizinga

Cockayne Syndrome: Varied Requirement of Transcription-Coupled Nucleotide Excision Repair for the Removal of Three Structurally Different Adducts from Transcribed DNA

Nataliya Kitsera, Karola Gasteiger, [ ... ], Andriy Khobta

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