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Vector construction

A Simplified and Versatile System for the Simultaneous Expression of Multiple siRNAs in Mammalian Cells Using Gibson DNA Assembly

Fang Deng, Xiang Chen, [ ... ], Tong-Chuan He

Rapid Restriction Enzyme-Free Cloning of PCR Products: A High-Throughput Method Applicable for Library Construction

Vijay K. Chaudhary, Nimisha Shrivastava, [ ... ], Amita Gupta

The HBx Oncoprotein of Hepatitis B Virus Deregulates the Cell Cycle by Promoting the Intracellular Accumulation and Re-Compartmentalization of the Cellular Deubiquitinase USP37

Nehul Saxena, Vijay Kumar

Analysis of the Role of Homology Arms in Gene-Targeting Vectors in Human Cells

Ayako Ishii, Aya Kurosawa, Shinta Saito, Noritaka Adachi

IRDL Cloning: A One-Tube, Zero-Background, Easy-to-Use, Directional Cloning Method Improves Throughput in Recombinant DNA Preparation

Jiancai Wang, Ronghua Xu, Aizhong Liu

HomeRun Vector Assembly System: A Flexible and Standardized Cloning System for Assembly of Multi-Modular DNA Constructs

Ming V. Li, Dip Shukla, [ ... ], David A. Largaespada

Role of Intron-Mediated Enhancement on Accumulation of an Arabidopsis NB-LRR Class R-protein that Confers Resistance to Cucumber mosaic virus

Yukiyo Sato, Sugihiro Ando, Hideki Takahashi

A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering

Anne Mathilde Lund, Helene Faustrup Kildegaard, [ ... ], Uffe Hasbro Mortensen

An Upstream Open Reading Frame Regulates LST1 Expression during Monocyte Differentiation

Christian Schiller, Carina Nowak, [ ... ], Elisabeth H. Weiss

Cockayne Syndrome: Varied Requirement of Transcription-Coupled Nucleotide Excision Repair for the Removal of Three Structurally Different Adducts from Transcribed DNA

Nataliya Kitsera, Karola Gasteiger, [ ... ], Andriy Khobta

Tools for Gene-Regulatory Analyses in the Marine Annelid Platynereis dumerilii

Benjamin Backfisch, Vitaly V. Kozin, [ ... ], Florian Raible

A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants

Andreas Binder, Jayne Lambert, [ ... ], Martin Parniske

Novel Method to Load Multiple Genes onto a Mammalian Artificial Chromosome

Anna Tóth, Katalin Fodor, [ ... ], Robert L. Katona

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