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Vector construction

A Simplified and Versatile System for the Simultaneous Expression of Multiple siRNAs in Mammalian Cells Using Gibson DNA Assembly

Fang Deng, Xiang Chen, Zhan Liao, Zhengjian Yan, Zhongliang Wang, Youlin Deng, Qian Zhang, Zhonglin Zhang, Jixing Ye, Min Qiao, Ruifang Li, Sahitya Denduluri, Jing Wang, Qiang Wei, Melissa Li, Nisha Geng, Lianggong Zhao, Guolin Zhou, Penghui Zhang, Hue H. Luu, Rex C. Haydon, Russell R. Reid, Tian Yang, Tong-Chuan He

published 14 Nov 2014

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Rapid Restriction Enzyme-Free Cloning of PCR Products: A High-Throughput Method Applicable for Library Construction

Vijay K. Chaudhary, Nimisha Shrivastava, Vaishali Verma, Shilpi Das, Charanpreet Kaur, Payal Grover, Amita Gupta

published 31 Oct 2014

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The HBx Oncoprotein of Hepatitis B Virus Deregulates the Cell Cycle by Promoting the Intracellular Accumulation and Re-Compartmentalization of the Cellular Deubiquitinase USP37

Nehul Saxena, Vijay Kumar

published 27 Oct 2014

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Analysis of the Role of Homology Arms in Gene-Targeting Vectors in Human Cells

Ayako Ishii, Aya Kurosawa, Shinta Saito, Noritaka Adachi

published 24 Sep 2014

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IRDL Cloning: A One-Tube, Zero-Background, Easy-to-Use, Directional Cloning Method Improves Throughput in Recombinant DNA Preparation

Jiancai Wang, Ronghua Xu, Aizhong Liu

published 22 Sep 2014

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HomeRun Vector Assembly System: A Flexible and Standardized Cloning System for Assembly of Multi-Modular DNA Constructs

Ming V. Li, Dip Shukla, Brian H. Rhodes, Anjali Lall, Jingmin Shu, Branden S. Moriarity, David A. Largaespada

published 24 Jun 2014

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Role of Intron-Mediated Enhancement on Accumulation of an Arabidopsis NB-LRR Class R-protein that Confers Resistance to Cucumber mosaic virus

Yukiyo Sato, Sugihiro Ando, Hideki Takahashi

published 10 Jun 2014

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A Versatile System for USER Cloning-Based Assembly of Expression Vectors for Mammalian Cell Engineering

Anne Mathilde Lund, Helene Faustrup Kildegaard, Maja Borup Kjær Petersen, Julie Rank, Bjarne Gram Hansen, Mikael Rørdam Andersen, Uffe Hasbro Mortensen

published 30 May 2014

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An Upstream Open Reading Frame Regulates LST1 Expression during Monocyte Differentiation

Christian Schiller, Carina Nowak, Kalliope N. Diakopoulos, Ulrich H. Weidle, Elisabeth H. Weiss

published 09 May 2014

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Cockayne Syndrome: Varied Requirement of Transcription-Coupled Nucleotide Excision Repair for the Removal of Three Structurally Different Adducts from Transcribed DNA

Nataliya Kitsera, Karola Gasteiger, Bork Lühnsdorf, Julia Allgayer, Bernd Epe, Thomas Carell, Andriy Khobta

published 08 Apr 2014

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Tools for Gene-Regulatory Analyses in the Marine Annelid Platynereis dumerilii

Benjamin Backfisch, Vitaly V. Kozin, Stephan Kirchmaier, Kristin Tessmar-Raible, Florian Raible

published 08 Apr 2014

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A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants

Andreas Binder, Jayne Lambert, Robert Morbitzer, Claudia Popp, Thomas Ott, Thomas Lahaye, Martin Parniske

published 13 Feb 2014

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Novel Method to Load Multiple Genes onto a Mammalian Artificial Chromosome

Anna Tóth, Katalin Fodor, Tünde Praznovszky, Vilmos Tubak, Andor Udvardy, Gyula Hadlaczky, Robert L. Katona

published 15 Jan 2014

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