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Vector construction

A Modular Plasmid Assembly Kit for Multigene Expression, Gene Silencing and Silencing Rescue in Plants

Andreas Binder, Jayne Lambert, [ ... ], Martin Parniske

High-Throughput Construction of Intron-Containing Hairpin RNA Vectors for RNAi in Plants

Pu Yan, Wentao Shen, [ ... ], Jun Duan

Assembly of Designer TAL Effectors by Golden Gate Cloning

Ernst Weber, Ramona Gruetzner, [ ... ], Sylvestre Marillonnet

Golden GATEway Cloning – A Combinatorial Approach to Generate Fusion and Recombination Constructs

Stephan Kirchmaier, Katharina Lust, Joachim Wittbrodt

Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1’ site

Song Yang, Ye Kuang, [ ... ], Donghai Wu

Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha

Shigeo S. Sugano, Ryuichi Nishihama, [ ... ], Takayuki Kohchi

Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly

Andreas I. Andreou, Naomi Nakayama

Analysis of the Role of Homology Arms in Gene-Targeting Vectors in Human Cells

Ayako Ishii, Aya Kurosawa, Shinta Saito, Noritaka Adachi

In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization

Xinglin Jiang, Jianming Yang, [ ... ], Mo Xian

High-Throughput Functional MicroRNAs Profiling by Recombinant AAV-Based MicroRNA Sensor Arrays

Wenhong Tian, Xiaoyan Dong, [ ... ], Xiaobing Wu

Strength and Regulation of Seven rRNA Promoters in Escherichia coli

Michihisa Maeda, Tomohiro Shimada, Akira Ishihama

A Modular Toolset for Recombination Transgenesis and Neurogenetic Analysis of Drosophila

Ji-Wu Wang, Erin S. Beck, Brian D. McCabe

Relative Quantification of Protein-Protein Interactions Using a Dual Luciferase Reporter Pull-Down Assay System

Shuaizheng Jia, Jianchun Peng, [ ... ], Linsheng Zhan

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