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Synthetic genomics

CRISPR genome editing using a combined positive and negative selection system

Ishrya Sharma, Kerisa Hall, Shannon Moonah

A simple validation and screening method for CRISPR/Cas9-mediated gene editing in mouse embryos to facilitate genetically modified mice production

Dawid Winiarczyk, Hossein Khodadadi, Paweł Leszczyński, Hiroaki Taniguchi

Unveiling the endogenous CRISPR-Cas system in Pseudomonas aeruginosa PAO1

Javier Alejandro Delgado-Nungaray, Luis Joel Figueroa-Yáñez, [ ... ], Orfil Gonzalez-Reynoso

Evidence for gene essentiality in Leishmania using CRISPR

Wen-Wei Zhang, Greg Matlashewski

GO-CRISPR: A highly controlled workflow to discover gene essentiality in loss-of-function screens

Pirunthan Perampalam, James I. McDonald, Frederick A. Dick

Development of an optimized protocol for generating knockout cancer cell lines using the CRISPR/Cas9 system, with emphasis on transient transfection

Seyed Alireza Mousavi Kahaki, Nayereh Ebrahimzadeh, Hossein Fahimi, Arfa Moshiri

CRISPR is easy: Exposure to Last Week Tonight enhances knowledge about gene editing

April A. Eichmeier, Michael A. Xenos

Hit screening with multivariate robust outlier detection

Hui Sun Leong, Tianhui Zhang, [ ... ], Steven Novick

A statistical simulation model to guide the choices of analytical methods in arrayed CRISPR screen experiments

Chang Sik Kim, Jonathan Cairns, [ ... ], Xiang Zhang

Method for isolation of high molecular weight genomic DNA from Botryococcus biomass

Ivette Cornejo-Corona, Devon J. Boland, Timothy P. Devarenne

A PLA₂ deletion mutant using CRISPR/Cas9 coupled to RNASeq reveals insect immune genes associated with eicosanoid signaling

Mohammad Vatanparast, Mojtaba Esmaeily, David Stanley, Yonggyun Kim

Zygote cryobanking applied to CRISPR/Cas9 microinjection in mice

Geraldine Schlapp, María Noel Meikle, [ ... ], Martina Crispo

A targeted CRISPR-Cas9 mediated F0 screen identifies genes involved in establishment of the enteric nervous system

Rodrigo Moreno-Campos, Eileen W. Singleton, Rosa A. Uribe

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