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Subcloning

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

Carola Engler, Romy Kandzia, Sylvestre Marillonnet

A simple and dual expression plasmid system in prokaryotic (E. coli) and mammalian cells

Manabu Murakami, Takayoshi Ohba, [ ... ], Shirou Itagaki

An Integrated Transcriptomic and Meta-Analysis of Hepatoma Cells Reveals Factors That Influence Susceptibility to HCV Infection

Jamie I. MacPherson, Ben Sidders, [ ... ], David L. Robertson

An Agar-Based Method for Plating Marine Protozoan Parasites of the Genus Perkinsus

Emma R. Cold, Nastasia J. Freyria, Joaquín Martínez Martínez, José A. Fernández Robledo

A dual prokaryotic (E. coli) expression system (pdMAX)

Manabu Murakami, Agnieszka M. Murakami, Shirou Itagaki

Lambda Red Mediated Gap Repair Utilizes a Novel Replicative Intermediate in Escherichia coli

Thimma R. Reddy, Léna M. S. Fevat, [ ... ], Shaun M. Cowley

A Rapid Cloning Method Employing Orthogonal End Protection

Arjen J. Jakobi, Eric G. Huizinga

Automated Solid-Phase Subcloning Based on Beads Brought into Proximity by Magnetic Force

Elton P. Hudson, Andrej Nikoshkov, Mathias Uhlen, Johan Rockberg

Functional Expression of Human NKCC1 from a Synthetic Cassette-Based cDNA: Introduction of Extracellular Epitope Tags and Removal of Cysteines

Suma Somasekharan, Michelle Y. Monette, Biff Forbush

Differential Requirements of Singleplex and Multiplex Recombineering of Large DNA Constructs

Thimma R. Reddy, Emma J. Kelsall, [ ... ], Shaun M. Cowley

Nickel-Resistance Determinants in Acidiphilium sp. PM Identified by Genome-Wide Functional Screening

Patxi San Martin-Uriz, Salvador Mirete, [ ... ], Jose E. Gonzalez-Pastor

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