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Sequencing techniques

Identification and verification of seed development related miRNAs in kernel almond by small RNA sequencing and qPCR

Marjan Jafari, Behrouz Shiran, Gholamreza Rabiei, Roudabeh Ravash, Badraldin Ebrahim Sayed Tabatabaei, Pedro Martínez-Gómez

published 01 Dec 2021

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Identification of individual root-knot nematodes using low coverage long-read sequencing

Graham S. Sellers, Daniel C. Jeffares, Bex Lawson, Tom Prior, David H. Lunt

published 01 Dec 2021

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Transcriptome sequencing revealed the influence of blue light on the expression levels of light-stress response genes in Centella asiatica

Wanapinun Nawae, Thippawan Yoocha, Nattapol Narong, Atchara Paemanee, Yanisa Ketngamkum, Kanokwan Romyanon, Theerayut Toojinda, Sithichoke Tangphatsornruang, Wirulda Pootakham

published 29 Nov 2021

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Rapid molecular diagnosis of Parechovirus infection using the reverse transcription loop-mediated isothermal amplification technique

Tadafumi Yokoyama, Yuko Tasaki, Natsumi Inoue, Naotoshi Sugimoto, Eri Nariai, Sanae Kuramoto, Taizo Wada

published 29 Nov 2021

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DNA bridges: A novel platform for single-molecule sequencing and other DNA-protein interaction applications

Maurizio Righini, Justin Costa, Wei Zhou

published 22 Nov 2021

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The EIF4E1-4EIP cap-binding complex of Trypanosoma brucei interacts with the terminal uridylyl transferase TUT3

Franziska Falk, Kevin Kamanyi Marucha, Christine Clayton

published 22 Nov 2021

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Porcine reproductive and respiratory syndrome virus 2 (PRRSV-2) genetic diversity and occurrence of wild type and vaccine-like strains in the United States swine industry

Mariana Kikuti, Juan Sanhueza, Carles Vilalta, Igor Adolfo Dexheimer Paploski, Kimberly VanderWaal, Cesar A. Corzo

published 19 Nov 2021

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Transcriptome profiling based on Illumina- and SMRT-based RNA-seq reveals circadian regulation of key pathways in flower bud development in walnut

Kai Ma, Xiang Luo, Liqun Han, Yu Zhao, Aisajan Mamat, Ning Li, Chuang Mei, Peng Yan, Rui Zhang, Jianfang Hu, Jixun Wang

published 18 Nov 2021

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Extraction and high-throughput sequencing of oak heartwood DNA: Assessing the feasibility of genome-wide DNA methylation profiling

Federico Rossi, Alessandro Crnjar, Federico Comitani, Rodrigo Feliciano, Leonie Jahn, George Malim, Laura Southgate, Emily Kay, Rebecca Oakey, Richard Buggs, Andy Moir, Logan Kistler, Ana Rodriguez Mateos, Carla Molteni, Reiner Schulz

published 18 Nov 2021

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Breadth of CD8 T-cell mediated inhibition of replication of diverse HIV-1 transmitted-founder isolates correlates with the breadth of recognition within a comprehensive HIV-1 Gag, Nef, Env and Pol potential T-cell epitope (PTE) peptide set

Peter Hayes, Natalia Fernandez, Christina Ochsenbauer, Jama Dalel, Jonathan Hare, Deborah King, Lucas Black, Claire Streatfield, Vanaja Kakarla, Gladys Macharia, Julia Makinde, Matt Price, Eric Hunter, The IAVI protocol C investigators , Jill Gilmour

published 17 Nov 2021

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An optimized method for RNA extraction from the polyurethane oligomer degrading strain Pseudomonas capeferrum TDA1 growing on aromatic substrates such as phenol and 2,4-diaminotoluene

María José Cárdenas Espinosa, Tabea Schmidgall, Georg Wagner, Uwe Kappelmeyer, Stephan Schreiber, Hermann J. Heipieper, Christian Eberlein

published 15 Nov 2021

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Use of universal primers for the 18S ribosomal RNA gene and whole soil DNAs to reveal the taxonomic structures of soil nematodes by high-throughput amplicon sequencing

Harutaro Kenmotsu, Emi Takabayashi, Akinori Takase, Yuu Hirose, Toshihiko Eki

published 15 Nov 2021

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Return of individual research results from genomic research: A systematic review of stakeholder perspectives

Danya F. Vears, Joel T. Minion, Stephanie J. Roberts, James Cummings, Mavis Machirori, Mwenza Blell, Isabelle Budin-Ljøsne, Lorraine Cowley, Stephanie O. M. Dyke, Clara Gaff, Robert Green, Alison Hall, Amber L. Johns, Bartha M. Knoppers, Stephanie Mulrine, Christine Patch, Eva Winkler, Madeleine J. Murtagh

published 08 Nov 2021

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