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RNA isolation

Optimizing direct RT-LAMP to detect transmissible SARS-CoV-2 from primary nasopharyngeal swab samples

Dawn M. Dudley, Christina M. Newman, Andrea M. Weiler, Mitchell D. Ramuta, Cecilia G. Shortreed, Anna S. Heffron, Molly A. Accola, William M. Rehrauer, Thomas C. Friedrich, David H. O’Connor

published 31 Dec 2020

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A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2

Tomislav Maricic, Olaf Nickel, Ayinuer Aximu-Petri, Elena Essel, Marie Gansauge, Philipp Kanis, Dominik Macak, Julia Richter, Stephan Riesenberg, Lukas Bokelmann, Hugo Zeberg, Matthias Meyer, Stephan Borte, Svante Pääbo

published 31 Dec 2020

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A simplified SARS-CoV-2 detection protocol for research laboratories

Sean Paz, Christopher Mauer, Anastasia Ritchie, Janet D. Robishaw, Massimo Caputi

published 18 Dec 2020

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Quality assessment of RNA in long-term storage: The All Our Families biorepository

Nikki L. Stephenson, Kylie K. Hornaday, Chelsea T. A. Doktorchik, Andrew W. Lyon, Suzanne C. Tough, Donna M. Slater

published 01 Dec 2020

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Detection of SARS-CoV-2 from raw patient samples by coupled high temperature reverse transcription and amplification

Johannes W. P. Kuiper, Timo Baade, Marcel Kremer, Ramon Kranaster, Linda Irmisch, Marcus Schuchmann, Johannes Zander, Andreas Marx, Christof R. Hauck

published 02 Nov 2020

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Rapid SARS-CoV-2 testing in primary material based on a novel multiplex RT-LAMP assay

Bernhard Schermer, Francesca Fabretti, Maximilian Damagnez, Veronica Di Cristanziano, Eva Heger, Sita Arjune, Nathan A. Tanner, Thomas Imhof, Manuel Koch, Alim Ladha, Julia Joung, Jonathan S. Gootenberg, Omar O. Abudayyeh, Volker Burst, Feng Zhang, Florian Klein, Thomas Benzing, Roman-Ulrich Müller

published 02 Nov 2020

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Heatr9 is an infection responsive gene that affects cytokine production in alveolar epithelial cells

Christopher J. Stairiker, Marjan van Meurs, Leticia G. Leon, A. A. Brouwers-Haspels, Laurine Rijsbergen, Yvonne M. Mueller, Peter D. Katsikis

published 17 Jul 2020

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RNA from stabilized whole blood enables more comprehensive immune gene expression profiling compared to RNA from peripheral blood mononuclear cells

Fleur van der Sijde, Yunlei Li, Rick Schraauwen, Willem de Koning, Casper H. J. van Eijck, Dana A. M. Mustafa

published 26 Jun 2020

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Rapid detection of novel coronavirus/Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by reverse transcription-loop-mediated isothermal amplification

Laura E. Lamb, Sarah N. Bartolone, Elijah Ward, Michael B. Chancellor

published 12 Jun 2020

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Investigating gene expression profiles of whole blood and peripheral blood mononuclear cells using multiple collection and processing methods

Aarti Gautam, Duncan Donohue, Allison Hoke, Stacy Ann Miller, Seshamalini Srinivasan, Bintu Sowe, Leanne Detwiler, Jesse Lynch, Michael Levangie, Rasha Hammamieh, Marti Jett

published 06 Dec 2019

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Cadherin-11 contributes to liver fibrosis induced by carbon tetrachloride

Mesias Pedroza, Sarah To, Jennifer Smith, Sandeep K. Agarwal

published 03 Jul 2019

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Spermatozoal large RNA content is associated with semen characteristics, sociodemographic and lifestyle factors

Enrica Bianchi, Kim Boekelheide, Mark Sigman, Joseph M. Braun, Melissa Eliot, Susan J. Hall, Edward Dere, Kathleen Hwang

published 23 May 2019

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Elimination of bacterial DNA during RNA isolation from sputum: Bashing bead vortexing is preferable over prolonged DNase treatment

Csilla Paska, Imre Barta, Orsolya Drozdovszky, Balazs Antus

published 28 Mar 2019

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