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RNA extraction

Cracking it - successful mRNA extraction for digital gene expression analysis from decalcified, formalin-fixed and paraffin-embedded bone tissue

Alireza Saraji, Anne Offermann, Janine Stegmann-Frehse, Katharina Hempel, Duan Kang, Rosemarie Krupar, Christian Watermann, Danny Jonigk, Mark Philipp Kühnel, Jutta Kirfel, Sven Perner, Verena Sailer

published 16 Sep 2021

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Resilient SARS-CoV-2 diagnostics workflows including viral heat inactivation

Maria Jose Lista, Pedro M. Matos, Thomas J. A. Maguire, Kate Poulton, Elena Ortiz-Zapater, Robert Page, Helin Sertkaya, Ana M. Ortega-Prieto, Edward Scourfield, Aoife M. O’Byrne, Clement Bouton, Ruth E. Dickenson, Mattia Ficarelli, Jose M. Jimenez-Guardeño, Mark Howard, Gilberto Betancor, Rui Pedro Galao, Suzanne Pickering, Adrian W. Signell, Harry Wilson, Penelope Cliff, Mark Tan Kia Ik, Amita Patel, Eithne MacMahon, Emma Cunningham, Katie Doores, Monica Agromayor, Juan Martin-Serrano, Esperanza Perucha, Hannah E. Mischo, Manu Shankar-Hari, Rahul Batra, Jonathan Edgeworth, Mark Zuckerman, Michael H. Malim, Stuart Neil, Rocio Teresa Martinez-Nunez

published 15 Sep 2021

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Self-collection and pooling of samples as resources-saving strategies for RT-PCR-based SARS-CoV-2 surveillance, the example of travelers in French Polynesia

Maite Aubry, Iotefa Teiti, Anita Teissier, Vaea Richard, Teheipuaura Mariteragi-Helle, Kiyojiken Chung, Farah Deen, Tuterarii Paoaafaite, Van-Mai Cao-Lormeau

published 02 Sep 2021

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Development of an alternative saliva test for diagnosis of SARS-CoV-2 using TRIzol: Adapting to countries with lower incomes looking for a large-scale detection program

Haydee Miranda-Ortiz, Edith A. Fernández-Figueroa, Erika B. Ruíz-García, Anallely Muñoz-Rivas, Alejandra Méndez-Pérez, Jorge Méndez-Galván, Horacio Astudillo-de la Vega, Belem Gabiño-López, Ricardo Nava-Monroy, Alejandro López-Flores a la Torre, Tanit López-Vergara Anaya, Diana Vilar-Compte, Uriel Coquis-Navarrete, Melissa Valdés-Reyes, Sokani Sánchez-Montes, Ingeborg Becker

published 18 Aug 2021

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Robotic RNA extraction for SARS-CoV-2 surveillance using saliva samples

Jennifer R. Hamilton, Elizabeth C. Stahl, Connor A. Tsuchida, Enrique Lin-Shiao, C. Kimberly Tsui, Kathleen Pestal, Holly K. Gildea, Lea B. Witkowsky, Erica A. Moehle, Shana L. McDevitt, Matthew McElroy, Amanda Keller, Iman Sylvain, Ariana Hirsh, Alison Ciling, Alexander J. Ehrenberg, Bradley R. Ringeisen, Garth Huberty, Fyodor D. Urnov, Petros Giannikopoulos, Jennifer A. Doudna, IGI SARS-CoV-2 Consortium

published 05 Aug 2021

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EUAdb: A resource for COVID-19 test development and comparison

Alyssa Woronik, Henry W. Shaffer, Karin Kiontke, Jon M. Laurent, Ronald Zambrano, Mariah Daley, Jef D. Boeke, David H. A. Fitch

published 04 Aug 2021

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Supporting scale-up of COVID-19 RT-PCR testing processes with discrete event simulation

Jad El Hage, Patti Gravitt, Jacques Ravel, Nadia Lahrichi, Erica Gralla

published 29 Jul 2021

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Efficient method for isolation of high-quality RNA from Psidium guajava L. tissues

Paola de Avelar Carpinetti, Vinicius Sartori Fioresi, Thais Ignez da Cruz, Francine Alves Nogueira de Almeida, Drielli Canal, Adésio Ferreira, Marcia Flores da Silva Ferreira

published 26 Jul 2021

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SARS-CoV-2 PCR positivity rate and seroprevalence of related antibodies among a sample of patients in Cairo: Pre-wave 2 results of a screening program in a university hospital

Samia A. Girgis, Hala M. Hafez, Hoda Ezz Elarab, Basma Sherif, Moshira H. Sabry, Iman Afifi, Fatma Elzahraa Hassan, Amira Reda, Shaimaa Elsayed, Asmaa Mahmoud, Petra Habeb, Ihab S. Habil, Rasha S. Hussein, Isis M. Mossad, Ossama Mansour, Ashraf Omar, Ayman M. Saleh, Mahmoud El-Meteini

published 15 Jul 2021

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Implementation of an open-source robotic platform for SARS-CoV-2 testing by real-time RT-PCR

José Luis Villanueva-Cañas, Eva Gonzalez-Roca, Aitor Gastaminza Unanue, Esther Titos, Miguel Julián Martínez Yoldi, Andrea Vergara Gómez, Joan Anton Puig-Butillé

published 14 Jul 2021

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Reference genes for mesangial cell and podocyte qPCR gene expression studies under high-glucose and renin-angiotensin-system blocker conditions

Nicole Dittrich Hosni, Ana Carolina Anauate, Mirian Aparecida Boim

published 09 Jul 2021

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Detection of SARs-CoV-2 in wastewater using the existing environmental surveillance network: A potential supplementary system for monitoring COVID-19 transmission

Salmaan Sharif, Aamer Ikram, Adnan Khurshid, Muhammad Salman, Nayab Mehmood, Yasir Arshad, Jamal Ahmed, Rana Muhammad Safdar, Lubna Rehman, Ghulam Mujtaba, Jaffar Hussain, Johar Ali, Mehar Angez, Muhammad Masroor Alam, Ribqa Akthar, Muhammad Wasif Malik, Mirza Zeeshan Iqbal Baig, Muhammad Suleman Rana, Muhammad Usman, Muhammad Qaisar Ali, Abdul Ahad, Nazish Badar, Massab Umair, Sana Tamim, Asiya Ashraf, Faheem Tahir, Nida Ali

published 29 Jun 2021

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DRUL for school: Opening Pre-K with safe, simple, sensitive saliva testing for SARS-CoV-2

Mayu O. Frank, Nathalie E. Blachere, Salina Parveen, Ezgi Hacisuleyman, John Fak, Joseph M. Luna, Eleftherios Michailidis, Samara Wright, Pamela Stark, Ann Campbell, Ashley Foo, Thomas P. Sakmar, Virginia Huffman, Marissa Bergh, Audrey Goldfarb, Andres Mansisidor, Agata L. Patriotis, Karl H. Palmquist, Nicolas Poulton, Rachel Leicher, César D. M. Vargas, Irene Duba, Arlene Hurley, Joseph Colagreco, Nicole Pagane, Dana E. Orange, Kevin Mora, Jennifer L. Rakeman, Randal C. Fowler, Helen Fernandes, Michelle F. Lamendola-Essel, Nicholas Didkovsky, Leopolda Silvera, Joseph Masci, Machelle Allen, Charles M. Rice, Robert B. Darnell

published 25 Jun 2021

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