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RNA extraction

Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study

Masaki Karino, Mizuki Harada, Chihiro Yamada, Kyoko Fukuoka, Megumi Sugo, Hiroyuki Hanada, Daisaku Masuda, Shingo Adachi, Shota Nakao, Masayuki Seki, Masaya Yamato, Shizuya Yamashita, Toru Takano

published 14 Dec 2021

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Identification of the best housekeeping gene for RT-qPCR analysis of human pancreatic organoids

Alessandro Cherubini, Francesco Rusconi, Lorenza Lazzari

published 08 Dec 2021

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Rapid molecular diagnosis of Parechovirus infection using the reverse transcription loop-mediated isothermal amplification technique

Tadafumi Yokoyama, Yuko Tasaki, Natsumi Inoue, Naotoshi Sugimoto, Eri Nariai, Sanae Kuramoto, Taizo Wada

published 29 Nov 2021

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Probing Loop-Mediated Isothermal Amplification (LAMP) targeting two gene-fragments of rose rosette virus

Andrea Salazar, Francisco M. Ochoa-Corona, Jennifer D. Olson, Binoy Babu, Mathews Paret

published 29 Nov 2021

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Validation of a novel molecular assay to the diagnostic of COVID-19 based on real time PCR with high resolution melting

Beatriz Iandra da Silva Ferreira, Natália Lins da Silva-Gomes, Wagner Luis da Costa Nunes Pimentel Coelho, Vanessa Duarte da Costa, Vanessa Cristine de Souza Carneiro, Rafael Lopes Kader, Marisa Pimentel Amaro, Lívia Melo Villar, Fábio Miyajima, Soniza Vieira Alves-Leon, Vanessa Salete de Paula, Luciane Almeida Amado Leon, Otacilio Cruz Moreira

published 22 Nov 2021

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An optimized method for RNA extraction from the polyurethane oligomer degrading strain Pseudomonas capeferrum TDA1 growing on aromatic substrates such as phenol and 2,4-diaminotoluene

María José Cárdenas Espinosa, Tabea Schmidgall, Georg Wagner, Uwe Kappelmeyer, Stephan Schreiber, Hermann J. Heipieper, Christian Eberlein

published 15 Nov 2021

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Evaluation of a bioaerosol sampler for indoor environmental surveillance of Severe Acute Respiratory Syndrome Coronavirus 2

Patrick Finn Horve, Leslie Dietz, Dale Northcutt, Jason Stenson, Kevin Van Den Wymelenberg

published 15 Nov 2021

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LuNER: Multiplexed SARS-CoV-2 detection in clinical swab and wastewater samples

Elizabeth C. Stahl, Allan R. Gopez, Connor A. Tsuchida, Vinson B. Fan, Erica A. Moehle, Lea B. Witkowsky, Jennifer R. Hamilton, Enrique Lin-Shiao, Matthew McElroy, Shana L. McDevitt, Alison Ciling, C. Kimberly Tsui, Kathleen Pestal, Holly K. Gildea, Amanda Keller, Iman A. Sylvain, Clara Williams, Ariana Hirsh, Alexander J. Ehrenberg, Rose Kantor, Matthew Metzger, Kara L. Nelson, Fyodor D. Urnov, Bradley R. Ringeisen, Petros Giannikopoulos, Jennifer A. Doudna, IGI Testing Consortium

published 10 Nov 2021

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Direct detection of polioviruses using a recombinant poliovirus receptor

Nancy Gerloff, Mark Mandelbaum, Hong Pang, Nikail Collins, Brittani Brown, Hong Sun, Chelsea Harrington, Jessica Hecker, Chadi Agha, Cara C. Burns, Everardo Vega

published 02 Nov 2021

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Assessing a chip based rapid RTPCR test for SARS CoV-2 detection (TrueNat assay): A diagnostic accuracy study

Ujjala Ghoshal, Atul Garg, Shruthi Vasanth, Akshay K. Arya, Ankita Pandey, Nidhi Tejan, Vikas Patel, Vikram P. Singh

published 13 Oct 2021

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Comparison of methods for pre-processing, exosome isolation, and RNA extraction in unpasteurized bovine and human milk

Sanoji Wijenayake, Shafinaz Eisha, Zoya Tawhidi, Michael A. Pitino, Michael A. Steele, Alison S. Fleming, Patrick O. McGowan

published 30 Sep 2021

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Protocol for safe, affordable, and reproducible isolation and quantitation of SARS-CoV-2 RNA from wastewater

Monica Trujillo, Kristen Cheung, Anna Gao, Irene Hoxie, Sherin Kannoly, Nanami Kubota, Kaung Myat San, Davida S. Smyth, John J. Dennehy

published 23 Sep 2021

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WHotLAMP: A simple, inexpensive, and sensitive molecular test for the detection of SARS-CoV-2 in saliva

David Ng, Ana Pinharanda, Merly C. Vogt, Ashok Litwin-Kumar, Kyle Stearns, Urvashi Thopte, Enrico Cannavo, Armen Enikolopov, Felix Fiederling, Stylianos Kosmidis, Barbara Noro, Ines Rodrigues-Vaz, Hani Shayya, Peter Andolfatto, Darcy S. Peterka, Tanya Tabachnik, Jeanine D’Armiento, Monica Goldklang, Andres Bendesky

published 16 Sep 2021

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