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RNA extraction

Validation of new equipment for SARS-CoV-2 diagnosis in Ecuador: Detection of the virus and antibodies generated by disease and vaccines with one POC device

Stephany D. Villota, Eliana Veloz-Villavicencio, Santiago Garcia-Iturralde, Johanna Valentina Arévalo, Suying Lu, Katariina Jaenes, Yuxiu Guo, Seray Cicek, Karen Colwill, Anne-Claude Gingras, Rod Bremner, Patricio Ponce, Keith Pardee, Varsovia Enid Cevallos

published 16 Apr 2025

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mRNA extracted from frozen buffy coat samples stored long term in tubes with no RNA preservative shows promise for downstream sequencing analyses

Erik Bovinder Ylitalo, Linda Vidman, Sophia Harlid, Bethany Van Guelpen

published 19 Mar 2025

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Surfaces environmental monitoring of SARS-CoV-2: Loop mediated isothermal amplification (LAMP) and droplet digital PCR (ddPCR) in comparison with standard Reverse-Transcription quantitative polymerase chain reaction (RT-qPCR) techniques

Simona Spiteri, Irene Salamon, Luna Girolamini, Maria Rosaria Pascale, Federica Marino, Carlo Derelitto, Laura Caligaris, Simone Paghera, Manuela Ferracin, Sandra Cristino

published 03 Feb 2025

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Detection of SARS-CoV-2 and a possible variant in shelter cats

Ogi Okwumabua, Nancy Bradley-Siemens, Catherine Cruz, Lauren Chittick, Melissa Thompson

published 13 Jan 2025

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Optimizing RNA extraction methods for high-throughput transcriptome sequencing of formalin-fixed paraffin-embedded cardiac tissue specimens

Nkechi Martina Odogwu, Jin Sung Jang, Sabrina Albertson, Clinton Hagen, Boyd Rasmussen, Oommen Saji, Timothy J. Nelson, the Wanek Program Preclinical Pipeline

published 26 Dec 2024

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Assessment of salivary microRNA by RT-qPCR: Facing challenges in data interpretation for clinical diagnosis

Marc Van Der Hofstadt, Anna Cardinal, Morgane Lepeltier, Jérémy Boulestreau, Alimata Ouedraogo, Malik Kahli, Pierre Champigneux, Laurence Molina, Franck Molina, Thi Nhu Ngoc Van

published 10 Dec 2024

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Exploring the diagnostic potential of miRNA signatures in the Fabry disease serum: A comparative study of automated and manual sample isolations

Josephine Y. Fang, Saravanan Ayyadurai, Alyssa F. Pybus, Hiroshi Sugimoto, Mark G. Qian

published 28 Oct 2024

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A reverse transcription loop-mediated isothermal amplification assay for quick detection of tomato mosaic virus

Phostine M. Kirasi, Elijah M. Ateka, Edith K. Avedi, Hillary K. Yegon, Bramwel W. Wanjala, Hanu R. Pappu

published 13 Jun 2024

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Establishment of dual reverse transcriptase-polymerase chain reaction for detection system for Areca palm velarivirus 1

Chunlin Peng, Benyi Fan, Hui Zhu, Liyun Liu, Zhengwu Zhao, Liyun Huang

published 05 Jun 2024

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Evaluation of short-term hair follicle storage conditions for maintenance of RNA integrity

Eilís E. Harkin, John A. Browne, Barbara A. Murphy

published 31 May 2024

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Efficient wastewater sample filtration improves the detection of SARS-CoV-2 variants: An extensive analysis based on sequencing parameters

Angelo Robotto, Carlotta Olivero, Elisa Pozzi, Claudia Strumia, Camilla Crasà, Cristina Fedele, Maddalena Derosa, Massimo Di Martino, Stefania Latino, Giada Scorza, Andrea Civra, David Lembo, Paola Quaglino, Enrico Brizio, Denis Polato

published 24 May 2024

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Genetic diversity and distribution of noroviruses among all age groups of patients with diarrhea in Amhara National Regional State, Ethiopia

Dessie Tegegne, Aschalew Gelaw, Dawit Hailu Alemayehu, Tamrayehu Seyoum, Dereje Leta, Getachew Ferede, Andargachew Mulu, Baye Gelaw

published 21 May 2024

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Preanalytical considerations for clinical assays of circulating human miRNA-451a, miRNA-423-5p and miRNA-199a-3p for diagnostic purposes

Dinesh S. Chandel, Wesley A. Tom, Chao Jiang, Gary Krzyzanowski, Nirmalee Fernando, Appolinaire Olou, M. Rohan Fernando

published 20 May 2024

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