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RNA amplification

Evaluation of commercial DNA and RNA extraction methods for high-throughput sequencing of FFPE samples

Stine H. Kresse, Heidi M. Namløs, Susanne Lorenz, Jeanne-Marie Berner, Ola Myklebost, Bodil Bjerkehagen, Leonardo A. Meza-Zepeda

published 17 May 2018

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Detection of Zika virus using reverse-transcription LAMP coupled with reverse dot blot analysis in saliva

Maite Sabalza, Rubina Yasmin, Cheryl A. Barber, Talita Castro, Daniel Malamud, Beum Jun Kim, Hui Zhu, Richard A. Montagna, William R. Abrams

published 05 Feb 2018

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Single primer isothermal amplification (SPIA) combined with next generation sequencing provides complete bovine coronavirus genome coverage and higher sequence depth compared to sequence-independent single primer amplification (SISPA)

Mette Myrmel, Veslemøy Oma, Mamata Khatri, Hanne H. Hansen, Maria Stokstad, Mikael Berg, Anne-Lie Blomström

published 07 Nov 2017

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Rapid colorimetric detection of Zika virus from serum and urine specimens by reverse transcription loop-mediated isothermal amplification (RT-LAMP)

Amanda E. Calvert, Brad J. Biggerstaff, Nathan A. Tanner, Molly Lauterbach, Robert S. Lanciotti

published 25 Sep 2017

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Evaluation of a real-time method of simultaneous amplification and testing in diagnosis of Mycoplasma pneumoniae infection in children with pneumonia

Wei Li, You-hong Fang, Hong-qiang Shen, De-hua Yang, Qiang Shu, Shi-qiang Shang

published 16 May 2017

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Error baseline rates of five sample preparation methods used to characterize RNA virus populations

Jeffrey R. Kugelman, Michael R. Wiley, Elyse R. Nagle, Daniel Reyes, Brad P. Pfeffer, Jens H. Kuhn, Mariano Sanchez-Lockhart, Gustavo F. Palacios

published 09 Feb 2017

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Efficacy Assessment of Nucleic Acid Decontamination Reagents Used in Molecular Diagnostic Laboratories

Melina Fischer, Nathalie Renevey, Barbara Thür, Donata Hoffmann, Martin Beer, Bernd Hoffmann

published 13 Jul 2016

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A Rapid and Low-Cost PCR Thermal Cycler for Infectious Disease Diagnostics

Kamfai Chan, Pui-Yan Wong, Peter Yu, Justin Hardick, Kah-Yat Wong, Scott A. Wilson, Tiffany Wu, Zoe Hui, Charlotte Gaydos, Season S. Wong

published 12 Feb 2016

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Real-Time Reverse Transcription PCR Assay for Detection of Senecavirus A in Swine Vesicular Diagnostic Specimens

Alexa J. Bracht, Emily S. O’Hearn, Andrew W. Fabian, Roger W. Barrette, Abu Sayed

published 12 Jan 2016

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RNA Amplification Protocol Leads to Biased Polymerase Chain Reaction Results Especially for Low-Copy Transcripts of Human Bone Marrow-Derived Stromal Cells

Carolin Coenen, Stefanie Liedtke, Gesine Kogler

published 20 Oct 2015

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Cauliflower mosaic virus Transcriptome Reveals a Complex Alternative Splicing Pattern

Clément Bouton, Angèle Geldreich, Laëtitia Ramel, Lyubov A. Ryabova, Maria Dimitrova, Mario Keller

published 10 Jul 2015

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Duplex Real-Time RT-PCR Assays for the Detection and Typing of Epizootic Haemorrhagic Disease Virus

Cyril Viarouge, Emmanuel Breard, Stephan Zientara, Damien Vitour, Corinne Sailleau

published 10 Jul 2015

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Stability of HIV-1 Nucleic Acids in Dried Blood Spot Samples for HIV-1 Drug Resistance Genotyping

Susan C. Aitken, Carole L. Wallis, Wendy Stevens, Tobias Rinke de Wit, Rob Schuurman

published 06 Jul 2015

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