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Reverse transcriptase-polymerase chain reaction

Direct real-time RT-PCR for the detection of dengue virus from patient serum in Lao PDR

Vilayouth Phimolsannousith, Malavanh Vongsouvath, Padthana Kiedsathid, Manivanh Vongsouvath, Elizabeth A. Ashley, Audrey Dubot-Pérès

published 18 Aug 2025

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Diagnostic accuracy evaluation of a point-of-care antigen test for SARS-CoV-2 and influenza in UK primary care (RAPTOR-C19)

Thomas R. Fanshawe, Sharon Tonner, Philip J. Turner, Margaret Głogowska, Umasha Ukwatte, Cecilia Okusi, Jade Cogdale, Maria Zambon, Brian D. Nicholson, F. D. Richard Hobbs, Gail N. Hayward, on behalf of the RAPTOR-C19 Study Group

published 07 Aug 2025

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Open educational resources for distributed hands-on teaching in molecular biology

Ariel Cerda, Alejandro Aravena, Valentina Zapata, Anibal Arce, Wladimir Araya, Domingo Gallardo, Javiera Aviles, Francisco Quero, Isaac Nuñez, Tamara Matute, Felipe Navarro, Valentina Ferrando, Marta Blanco, Sebastian Velozo, Sebastian Rodriguez, Sebastian Aguilera, Francisco Chateau, Ruby Olivares-Donoso, Jennifer C. Molloy, Guy Aidelberg, Ariel B. Lindner, Fernando Castro, Pablo Cremades, Cesar Ramirez-Sarmiento, Fernan Federici

published 05 Aug 2025

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Development of isothermal nucleic acid amplification technologies for rapid detection of Porcine Enterovirus-G

Sarshti Kaushik, Sushila Maan, Kanisht Batra, Swati Sindhu, Vijay Kadian, Aman Kumar

published 02 Jul 2025

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Next-Generation Sequencing (NGS) in non-small cell lung carcinoma: A real-world experience in the public health system of Galicia (Northwest Spain)

Ihab Abdulkader-Nallib, José Manuel Cameselle-Teijeiro, Iván Lesende-Rodríguez, Raquel Pérez-Becerra, José Ramón Antúnez-López, Jorge García-González, Luis León-Mateos, María Sánchez-Ares

published 01 Jul 2025

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A targeted approach for multiplex detection of respiratory viruses in cases with severe acute respiratory infections by nanopore sequencing

Arghavan Zebardast, Kaveh Sadeghi, Ahmad Nejati, Sevrin Zadheidar, Mohammad Hossein Najmi, Adel Abedi, Vahid Salimi, Mehdi Shabani, Jila Yavarian, Nazanin Zahra Shafiei Jandaghi, Talat Mokhtari Azad

published 25 Jun 2025

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Assessment of a multiplex arbovirus PCR Detection Test in an area endemic for Chikungunya, Zika, and Dengue viruses: An evaluation of kit performance characteristics in line with Clinical Laboratory Improvement Amendments (CLIA) Standards

Leonard Kingwara, Lazarus Odeny, Vera Onwonga, Rukia Madada, Marygorett Mbeneka, Jully Okonji, Victoria Mwende, Charles Rombo, Shalyn Akasa, Millicent Ndia, Fredrick Ouma, Isabella Ayagah, Abdi Roba, Kamene Kimenye

published 24 Jun 2025

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Prevalence of dengue in febrile patients in Peru: A systematic review and meta-analysis

Darwin A. León-Figueroa, Edwin A. Garcia-Vasquez, Milagros Diaz-Torres, Edwin Aguirre-Milachay, Jean Pierre Villanueva-De La Cruz, Hortencia M. Saldaña-Cumpa, Mario J. Valladares-Garrido

published 17 Jun 2025

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Vaccine effectiveness of inactivated and mRNA COVID-19 vaccine platform during Delta and Omicron wave in Jakarta, Indonesia: A test-negative case-control study

Erlina Burhan, Farchan Azzumar, Fira Alyssa Gabriella Sinuraya, Sabarinah Prasetyo, Dwi Gayatri, Iwan Ariawan, Muhammad Ilham Dhiya Rakasiwi, Hanna Lianti Afladhia, Ahmad Fadhil Ilham, Ihya Akbar, Elvan Wiyarta

published 09 Jun 2025

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Detection of Deltacoronavirus in environmental fecal samples from seabirds in the Saint Peter and Saint Paul Archipelago, central equatorial Atlantic Ocean

Fernanda Gomes, Alexandre Freitas da Silva, Tatiana Prado, Marina Galvão Bueno, Luciana Appolinario, Patrícia Soares Flores, Paola Cristina Resende, Marilda Siqueira, Leonardo Corrêa, Martha Brandão, Jose Reck, Gabriel Luz Wallau, Maria Ogrzewalska

published 03 Jun 2025

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Beyond the ORF: Paralog-specific regulation of RPS7/eS7 mRNAs via 3’-UTRs and promoter sequences

Sachiko Hayashi, Tohru Yoshihisa

published 30 May 2025

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The added diagnostic value of RT-PCR on faeces for the diagnosis of COVID-19

Nathalie Van der Moeren, Rik van den Biggelaar, Karin B. Gast, Jaco J. Verweij, Barbara J.M. Bergmans, Joep J. J.M. Stohr, Jean-Luc Murk

published 29 May 2025

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Identification of stable reference genes in Edwardsiella ictaluri for accurate gene expression analysis

Jingjun Lu, Hossam Abdelhamed, Nawar Al-Janabi, Nour Eissa, Mark Lawrence, Attila Karsi

published 02 May 2025

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