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Purification techniques

Characterization of a cold-active, detergent-stable metallopeptidase purified from Bacillus sp. S1DI 10 using Response Surface Methodology

Drishtant Singh, Sharad Thakur, Seema Madhumal Thayil, Anup Kumar Kesavan

published 23 May 2019

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Optimizing the production and affinity purification of HIV-1 envelope glycoprotein SOSIP trimers from transiently transfected CHO cells

Albert Cupo, Victor M. Cruz Portillo, Paul Gelfand, Anila Yasmeen, P. J. Klasse, John P. Moore

published 08 Apr 2019

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Purification and biochemical characterization of FrsA protein from Vibrio vulnificus as an esterase

Xiaoqin Wang, Zhi-Min Li, Qingyue Li, Mingsong Shi, Lingling Bao, Dingguo Xu, Zhimin Li

published 05 Apr 2019

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EFCAB2 is a novel calcium-binding protein in mouse testis and sperm

Hossam H. Shawki, Yu Ishikawa-Yamauchi, Akihiro Kawashima, Yuki Katoh, Manabu Matsuda, Al-Sayed Al-Soudy, Fatma M. Minisy, Akihiro Kuno, Xiafukaiti Gulibaikelamu, Takatsugu Hirokawa, Satoru Takahashi, Hisashi Oishi

published 01 Apr 2019

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Engineering, and production of functionally active human Furin in N. benthamiana plant: In vivo post-translational processing of target proteins by Furin in plants

Tarlan Mamedov, Ilaha Musayeva, Rabia Acsora, Nilufer Gun, Burcu Gulec, Gulshan Mammadova, Kader Cicek, Gulnara Hasanova

published 12 Mar 2019

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Interaction between nectin-1 and the human natural killer cell receptor CD96

Veronica M. Holmes, Carlos Maluquer de Motes, Paige T. Richards, Jessenia Roldan, Arjun K. Bhargava, Jordan S. Orange, Claude Krummenacher

published 13 Feb 2019

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Expression optimization, purification, and functional characterization of cholesterol oxidase from Chromobacterium sp. DS1

Aliakbar Fazaeli, Abolfazl Golestani, Mostafa Lakzaei, Samaneh Sadat Rasi Varaei, Mahdi Aminian

published 13 Feb 2019

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An efficient and cost-effective method for purification of small sized DNAs and RNAs from human urine

Kayvan Zainabadi, Vaigundan Dhayabaran, Kutty Moideen, Patnam Krishnaswamy

published 05 Feb 2019

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Design, expression and functional characterization of a thermostable xylanase from Trichoderma reesei

Jun He, Feng Tang, Daiwen Chen, Bing Yu, Yuheng Luo, Ping Zheng, Xiangbing Mao, Jie Yu, Feng Yu

published 16 Jan 2019

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Characterization of novel lignocellulose-degrading enzymes from the porcupine microbiome using synthetic metagenomics

Mackenzie Thornbury, Jacob Sicheri, Patrick Slaine, Landon J. Getz, Emma Finlayson-Trick, Jamie Cook, Caroline Guinard, Nicholas Boudreau, David Jakeman, John Rohde, Craig McCormick

published 02 Jan 2019

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Filter paper-based spin column method for cost-efficient DNA or RNA purification

Rui Shi, Ramsey S. Lewis, Dilip R. Panthee

published 07 Dec 2018

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An improved DNA array-based classification method for the identification of Salmonella serotypes shows high concordance between traditional and genotypic testing

James Robertson, Catherine Yoshida, Simone Gurnik, Madison McGrogan, Kristin Davis, Gitanjali Arya, Stephanie A. Murphy, Anil Nichani, John H. E. Nash

published 04 Dec 2018

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Recombinant AfusinC, an anionic fungal CSαβ defensin from Aspergillus fumigatus, exhibits antimicrobial activity against gram-positive bacteria

Gabriela Contreras, Markus Santhosh Braun, Holger Schäfer, Michael Wink

published 11 Oct 2018

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