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Purification techniques

Phenol-chloroform-based RNA purification for detection of SARS-CoV-2 by RT-qPCR: Comparison with automated systems

Henrik Dimke, Sanne L. Larsen, Marianne N. Skov, Hanne Larsen, Gitte N. Hartmeyer, Jesper B. Moeller

published 24 Feb 2021

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23-valent polysaccharide vaccine (PPSV23)-targeted serotype-specific identification of Streptococcus pneumoniae using the loop-mediated isothermal amplification (LAMP) method

Jiwon Lee, Youngbae Yoon, Eun Jin Kim, Donghyun Lee, Yeongjun Baek, Chika Takano, Bin Chang, Takahiro Iijima, Paul E. Kilgore, Satoshi Hayakawa, Tomonori Hoshino, Dong Wook Kim, Mitsuko Seki

published 16 Feb 2021

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Rapid processing of SARS-CoV-2 containing specimens for direct RT-PCR

Piotr Chomczynski, Peter W. Chomczynski, Amy Kennedy, Michal Rymaszewski, William W. Wilfinger, Judith A. Heiny, Karol Mackey

published 10 Feb 2021

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Recombinase polymerase amplification assay combined with a dipstick-readout for rapid detection of Mycoplasma ovipneumoniae infections

Sandeep K. Gupta, Qing Deng, Tanushree B. Gupta, Paul Maclean, Joerg Jores, Axel Heiser, D. Neil Wedlock

published 04 Feb 2021

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Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp

Paula Finamore-Araujo, Amanda Faier-Pereira, Carlos Ramon do Nascimento Brito, Eldrinei Gomes Peres, Klenicy Kazumy de Lima Yamaguchi, Renata Trotta Barroso Ferreira, Otacilio Cruz Moreira

published 02 Feb 2021

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Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants

Thorben Detering, Katharina Mundry, Ralf G. Berger

published 21 Dec 2020

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Cloning, expression and purification of recombinant dermatopontin in Escherichia coli

Trikkur Madom Seetaraman Amritha, Shubham Mahajan, Kumar Subramaniam, Yamini Chandramohan, Anuradha Dhanasekaran

published 30 Nov 2020

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A highly active heparinase I from Bacteroides cellulosilyticus: Cloning, high level expression, and molecular characterization

Li-Wei Gao, Hong-Tao Zhu, Cai-Yun Liu, Zhi-Xiang Lv, Xiao-Man Fan, Ye-Wang Zhang

published 20 Oct 2020

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Kinetic and structural insights into enzymatic mechanism of succinic semialdehyde dehydrogenase from Cyanothece sp. ATCC51142

Congcong Xie, Zhi-Min Li, Fumei Bai, Ziwei Hu, Wei Zhang, Zhimin Li

published 23 Sep 2020

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First echinoderm alpha-amylase from a tropical sea cucumber (Holothuria leucospilota): Molecular cloning, tissue distribution, cellular localization and functional production in a heterogenous E.coli system with codon optimization

Xiaofen Wu, Yao Ruan, Ting Chen, Zonghe Yu, Da Huo, Xiaomin Li, Feifei Wu, Xiao Jiang, Chunhua Ren

published 15 Sep 2020

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Diversification of polyphosphate end-labeling via bridging molecules

Catherine J. Baker, Stephanie A. Smith, James H. Morrissey

published 21 Aug 2020

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Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics

Yiping Zou, Michael Glenn Mason, Jose Ramon Botella

published 29 Jun 2020

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Structural and biochemical characterization of a novel thermophilic Coh01147 protease

Hossein Tarrahimofrad, Amir Meimandipour, Sareh Arjmand, Mohammadtaghi Beigi Nassiri, Ehsan Jahangirian, Hossein Tavana, Javad Zamani, Somayyeh Rahimnahal, Saeed Aminzadeh

published 23 Jun 2020

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