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Purification techniques

Phenol-chloroform-based RNA purification for detection of SARS-CoV-2 by RT-qPCR: Comparison with automated systems

Henrik Dimke, Sanne L. Larsen, [ ... ], Jesper B. Moeller

23-valent polysaccharide vaccine (PPSV23)-targeted serotype-specific identification of Streptococcus pneumoniae using the loop-mediated isothermal amplification (LAMP) method

Jiwon Lee, Youngbae Yoon, [ ... ], Mitsuko Seki

Rapid processing of SARS-CoV-2 containing specimens for direct RT-PCR

Piotr Chomczynski, Peter W. Chomczynski, [ ... ], Karol Mackey

Recombinase polymerase amplification assay combined with a dipstick-readout for rapid detection of Mycoplasma ovipneumoniae infections

Sandeep K. Gupta, Qing Deng, [ ... ], D. Neil Wedlock

Validation of a novel multiplex real-time PCR assay for Trypanosoma cruzi detection and quantification in açai pulp

Paula Finamore-Araujo, Amanda Faier-Pereira, [ ... ], Otacilio Cruz Moreira

Generation of 4-vinylguaiacol through a novel high-affinity ferulic acid decarboxylase to obtain smoke flavours without carcinogenic contaminants

Thorben Detering, Katharina Mundry, Ralf G. Berger

Cloning, expression and purification of recombinant dermatopontin in Escherichia coli

Trikkur Madom Seetaraman Amritha, Shubham Mahajan, [ ... ], Anuradha Dhanasekaran

A highly active heparinase I from Bacteroides cellulosilyticus: Cloning, high level expression, and molecular characterization

Li-Wei Gao, Hong-Tao Zhu, [ ... ], Ye-Wang Zhang

Kinetic and structural insights into enzymatic mechanism of succinic semialdehyde dehydrogenase from Cyanothece sp. ATCC51142

Congcong Xie, Zhi-Min Li, [ ... ], Zhimin Li

First echinoderm alpha-amylase from a tropical sea cucumber (Holothuria leucospilota): Molecular cloning, tissue distribution, cellular localization and functional production in a heterogenous E.coli system with codon optimization

Xiaofen Wu, Yao Ruan, [ ... ], Chunhua Ren

Diversification of polyphosphate end-labeling via bridging molecules

Catherine J. Baker, Stephanie A. Smith, James H. Morrissey

Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics

Yiping Zou, Michael Glenn Mason, Jose Ramon Botella

Structural and biochemical characterization of a novel thermophilic Coh01147 protease

Hossein Tarrahimofrad, Amir Meimandipour, [ ... ], Saeed Aminzadeh

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