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Purification techniques

Importance of annexin V N-terminus for 2D crystal formation and quick purification protocol of recombinant annexin V

Ryusei Yamada, Tran Ngoc Trang, Holger Flechsig, Toshiki Takeda, Noriyuki Kodera, Hiroki Konno

published 22 Dec 2022

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Efficient production of protein complexes in mammalian cells using a poxvirus vector

Robert Drillien, Karine Pradeau-Aubreton, Julien Batisse, Joëlle Mezher, Emma Schenckbecher, Justine Marguin, Eric Ennifar, Marc Ruff

published 15 Dec 2022

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ATP hydrolytic activity of purified Spf1p correlate with micellar lipid fluidity and is dependent on conserved residues in transmembrane helix M1

Johan Ørskov Ipsen, Danny Mollerup Sørensen

published 20 Oct 2022

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Improved assessments of bulk milk microbiota composition via sample preparation and DNA extraction methods

Zhengyao Xue, Maria L. Marco

published 15 Sep 2022

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Multiplex real-time PCR using SYBR Green: Unspecific intercalating dye to detect antimicrobial resistance genes of Streptococcus pneumoniae in cerebrospinal fluid

Mariana Brena Souza, Maria Cecília Cergole-Novella, Delma Aparecida Molinari, Daniela Rodrigues Colpas, Andréia Moreira dos Santos Carmo, Vilma dos Santos Menezes Gaiotto Daros, Ivana Barros de Campos

published 14 Jun 2022

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Formation of recombinant bifunctional fusion protein: A newer approach to combine the activities of two enzymes in a single protein

Patel Nilpa, Kapadia Chintan, R. Z. Sayyed, Hesham El Enshasy, Hala El Adawi, Alaa Alhazmi, Atiah H. Almalki, Shafiul Haque

published 01 Apr 2022

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A novel approach for the purification of aggregation prone proteins

Austin Royster, Sheema Mir, Mohammad Ayoub Mir

published 22 Nov 2021

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Heterologous expression and purification of recombinant human protoporphyrinogen oxidase IX: A comparative study

Zora Novakova, Daria Khuntsaria, Marketa Gresova, Jana Mikesova, Barbora Havlinova, Shivam Shukla, Lucie Kolarova, Katerina Vesela, Pavel Martasek, Cyril Barinka

published 18 Nov 2021

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Long term conservation of DNA at ambient temperature. Implications for DNA data storage

Delphine Coudy, Marthe Colotte, Aurélie Luis, Sophie Tuffet, Jacques Bonnet

published 11 Nov 2021

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A new purification technique to obtain specific size distribution of giant lipid vesicles using dual filtration

Mohammad Abu Sayem Karal, Tawfika Nasrin, Marzuk Ahmed, Md. Kabir Ahamed, Shareef Ahammed, Salma Akter, Sharif Hasan, Zaid Bin Mahbub

published 29 Jul 2021

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Sperm-binding regions on bovine egg zona pellucida glycoprotein ZP4 studied in a solid supported form on plastic plate

Kamila Dilimulati, Misaki Orita, Ganbat Undram, Naoto Yonezawa

published 09 Jul 2021

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Extracellular proteolytic activation of Pseudomonas aeruginosa aminopeptidase (PaAP) and insight into the role of its non-catalytic N-terminal domain

Itschak Axelrad, Mary Safrin, Rivka Cahan, Sang-Jin Suh, Dennis E. Ohman, Efrat Kessler

published 16 Jun 2021

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Low saliva pH can yield false positives results in simple RT-LAMP-based SARS-CoV-2 diagnostic tests

Cristina Uribe-Alvarez, Quynh Lam, Don A. Baldwin, Jonathan Chernoff

published 05 May 2021

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