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Purification techniques

First echinoderm alpha-amylase from a tropical sea cucumber (Holothuria leucospilota): Molecular cloning, tissue distribution, cellular localization and functional production in a heterogenous E.coli system with codon optimization

Xiaofen Wu, Yao Ruan, [ ... ], Chunhua Ren

Site Directed Mutagenesis of Schizosaccharomyces pombe Glutathione Synthetase Produces an Enzyme with Homoglutathione Synthetase Activity

Tamara Dworeck, Martin Zimmermann

Expression of a β-Mannosidase from Paenibacillus polymyxa A-8 in Escherichia coli and Characterization of the Recombinant Enzyme

Xi Bai, Hong Hu, [ ... ], Qianming Huang

Optimizing and accelerating the assignation of lineages in Mycobacterium tuberculosis using novel alternative single-tube assays

María Carcelén, Estefanía Abascal, [ ... ], Darío García-de-Viedma

Purification and characterization of novel isoforms of the polyphenol oxidase from Malus domestica fruit pulp

Naila Sajjad, M. Sheeraz Ahmad, [ ... ], Dawood Ahmed

Polymorphisms of Mouse Apolipoprotein A-II Alter Its Physical and Functional Nature

Timothy J. Sontag, Catherine A. Reardon

Scalable Production of Recombinant Membrane Active Peptides and Its Potential as a Complementary Adjunct to Conventional Chemotherapeutics

Hussin A. Rothan, Jamunaa Ambikabothy, [ ... ], Rohana Yusof

Arg126 and Asp49 Are Essential for the Catalytic Function of Microsomal Prostaglandin E₂ Synthase 1 and Ser127 Is Not

Joan Raouf, Nazmi Rafique, [ ... ], Linda Spahiu

An inhibitor-free, versatile, fast, and cheap precipitation-based DNA purification method

Zhe F. Tang, David R. McMillen

A highly active heparinase I from Bacteroides cellulosilyticus: Cloning, high level expression, and molecular characterization

Li-Wei Gao, Hong-Tao Zhu, [ ... ], Ye-Wang Zhang

Bioorthogonal Non-Canonical Amino Acid Tagging (BONCAT) to detect newly synthesized proteins in cells and their secretome

Elizabeth P. Anim, Justin Mezzanotte, Siwei Chu, Ursula Stochaj

Enzymatic characterization and polyurethane biodegradation assay of two novel esterases isolated from a polluted river

Arianna Soto-Hernández, Luis Felipe Muriel-Millán, [ ... ], Liliana Pardo-López

Characterization of Protein Phosphatase 5 from Three Lepidopteran Insects: Helicoverpa armigera, Mythimna separata and Plutella xylostella

Xi’en Chen, Shumin Lü, Yalin Zhang

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