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Purification techniques

Efficient E. coli Expression Strategies for Production of Soluble Human Crystallin ALDH3A1

Georgia-Persephoni Voulgaridou, Theodora Mantso, Katerina Chlichlia, Mihalis I. Panayiotidis, Aglaia Pappa

published 22 Feb 2013

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Recombinant Production of Human Aquaporin-1 to an Exceptional High Membrane Density in Saccharomyces cerevisiae

Julie Bomholt, Claus Hélix-Nielsen, Peter Scharff-Poulsen, Per Amstrup Pedersen

published 11 Feb 2013

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Analysis of the Enzymatic Properties of a Broad Family of Alanine Aminotransferases

Chandra H. McAllister, Michelle Facette, Andrew Holt, Allen G. Good

published 07 Feb 2013

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Biochemical Similarities and Differences between the Catalytic [4Fe-4S] Cluster Containing Fumarases FumA and FumB from Escherichia coli

Barbara M. A. van Vugt-Lussenburg, Laura van der Weel, Wilfred R. Hagen, Peter-Leon Hagedoorn

published 06 Feb 2013

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Atypical Features of Thermus thermophilus Succinate:Quinone Reductase

Olga Kolaj-Robin, Mohamed R. Noor, Sarah R. O’Kane, Frauke Baymann, Tewfik Soulimane

published 07 Jan 2013

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The Molecular and Structural Characterization of Two Vitellogenins from the Free-Living Nematode Oscheius tipulae

Daniela P. Almenara, Joselene P. de Moura, Cristiane P. Scarabotto, Russolina B. Zingali, Carlos E. Winter

published 07 Jan 2013

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A Multiplexed Microfluidic PCR Assay for Sensitive and Specific Point-of-Care Detection of Chlamydia trachomatis

Deborah Dean, Rosemary S. Turingan, Hans-Ulrich Thomann, Anna Zolotova, James Rothschild, Sandeep J. Joseph, Timothy D. Read, Eugene Tan, Richard F. Selden

published 14 Dec 2012

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Self-Subunit Swapping Occurs in Another Gene Type of Cobalt Nitrile Hydratase

Yi Liu, Wenjing Cui, Yuanyuan Xia, Youtian Cui, Michihiko Kobayashi, Zhemin Zhou

published 30 Nov 2012

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Human Trifunctional Protein Alpha Links Cardiolipin Remodeling to Beta-Oxidation

William A. Taylor, Edgard M. Mejia, Ryan W. Mitchell, Patrick C. Choy, Genevieve C. Sparagna, Grant M. Hatch

published 09 Nov 2012

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Immobilized Metal Affinity Chromatography Co-Purifies TGF-β1 with Histidine-Tagged Recombinant Extracellular Proteins

Jasvir Kaur, Dieter P. Reinhardt

published 31 Oct 2012

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Site Directed Mutagenesis of Schizosaccharomyces pombe Glutathione Synthetase Produces an Enzyme with Homoglutathione Synthetase Activity

Tamara Dworeck, Martin Zimmermann

published 16 Oct 2012

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The PduQ Enzyme Is an Alcohol Dehydrogenase Used to Recycle NAD⁺ Internally within the Pdu Microcompartment of Salmonella enterica

Shouqiang Cheng, Chenguang Fan, Sharmistha Sinha, Thomas A. Bobik

published 15 Oct 2012

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GFP Facilitates Native Purification of Recombinant Perlucin Derivatives and Delays the Precipitation of Calcium Carbonate

Eva Weber, Christina Guth, Ingrid M. Weiss

published 03 Oct 2012

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