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Purification techniques

Mouse-Hamster Chimeric Prion Protein (PrP) Devoid of N-Terminal Residues 23-88 Restores Susceptibility to 22L Prions, but Not to RML Prions in PrP-Knockout Mice

Keiji Uchiyama, Hironori Miyata, Masashi Yano, Yoshitaka Yamaguchi, Morikazu Imamura, Naomi Muramatsu, Nandita Rani Das, Junji Chida, Hideyuki Hara, Suehiro Sakaguchi

published 16 Oct 2014

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A Rapid Method of Detecting Autoantibody against FcεRIα for Chronic Spontaneous Urticaria

Mey-Fann Lee, Tzu-Mei Lin, Szu-Wei Liu, Yi-Hsing Chen

published 15 Oct 2014

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Probing the Crucial Role of Leu31 and Thr33 of the Bacillus pumilus CBS Alkaline Protease in Substrate Recognition and Enzymatic Depilation of Animal Hide

Nadia Zaraî Jaouadi, Bassem Jaouadi, Hajer Ben Hlima, Hatem Rekik, Mouna Belhoul, Maher Hmidi, Houda Slimene Ben Aicha, Chiraz Gorgi Hila, Abdessatar Toumi, Nushin Aghajari, Samir Bejar

published 29 Sep 2014

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Evaluation of Inhibitory Action of Novel Non β-Lactam Inhibitor against Klebsiella pneumoniae Carbapenemase (KPC-2)

Arbab Khan, Mohammad Faheem, Mohd Danishuddin, Asad U. Khan

published 29 Sep 2014

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Semi-Automated Hydrophobic Interaction Chromatography Column Scouting Used in the Two-Step Purification of Recombinant Green Fluorescent Protein

Orrin J. Stone, Kelly M. Biette, Patrick J. M. Murphy

published 25 Sep 2014

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A Universal Approach to Prepare Reagents for DNA-Assisted Protein Analysis

Junhong Yan, Gucci Jijuan Gu, Christian Jost, Maria Hammond, Andreas Plückthun, Ulf Landegren, Masood Kamali-Moghaddam

published 18 Sep 2014

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Structural Basis for Substrate Specificity of Mammalian Neuraminidases

Victoria Smutova, Amgad Albohy, Xuefang Pan, Elena Korchagina, Taeko Miyagi, Nicolai Bovin, Christopher W. Cairo, Alexey V. Pshezhetsky

published 15 Sep 2014

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Characterization of a Soluble Phosphatidic Acid Phosphatase in Bitter Melon (Momordica charantia)

Heping Cao, Kandan Sethumadhavan, Casey C. Grimm, Abul H. J. Ullah

published 09 Sep 2014

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Characterization of the PH1704 Protease from Pyrococcus horikoshii OT3 and the Critical Functions of Tyr120

Dongling Zhan, Aixi Bai, Lei Yu, Weiwei Han, Yan Feng

published 05 Sep 2014

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A Naturally Occurring Lgr4 Splice Variant Encodes a Soluble Antagonist Useful for Demonstrating the Gonadal Roles of Lgr4 in Mammals

Pei-Jen Hsu, Fang-Ju Wu, Masataka Kudo, Chih-Lun Hsiao, Aaron J. W. Hsueh, Ching-Wei Luo

published 04 Sep 2014

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Detection of MPLW515L/K Mutations and Determination of Allele Frequencies with a Single-Tube PCR Assay

Hiraku Takei, Soji Morishita, Marito Araki, Yoko Edahiro, Yoshitaka Sunami, Yumi Hironaka, Naohiro Noda, Yuji Sekiguchi, Satoshi Tsuneda, Akimichi Ohsaka, Norio Komatsu

published 21 Aug 2014

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Domain Organization, Catalysis and Regulation of Eukaryotic Cystathionine Beta-Synthases

Tomas Majtan, Angel L. Pey, Roberto Fernández, José A. Fernández, Luis A. Martínez-Cruz, Jan P. Kraus

published 14 Aug 2014

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Kinetic and Sequence-Structure-Function Analysis of LinB Enzyme Variants with β- and δ-Hexachlorocyclohexane

Rinku Pandey, Del Lucent, Kirti Kumari, Pooja Sharma, Rup Lal, John G. Oakeshott, Gunjan Pandey

published 30 Jul 2014

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