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Protein expression

Design Parameters to Control Synthetic Gene Expression in Escherichia coli

Mark Welch, Sridhar Govindarajan, [ ... ], Claes Gustafsson

A Mass Spectrometric-Derived Cell Surface Protein Atlas

Damaris Bausch-Fluck, Andreas Hofmann, [ ... ], Bernd Wollscheid

Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins

David J. Leibly, Trang Nhu Nguyen, [ ... ], Wesley C. Van Voorhis

A new analysis method for evaluating bacterial growth with microplate readers

Venkata Rao Krishnamurthi, Isabelle I. Niyonshuti, Jingyi Chen, Yong Wang

Heterologous Expression of Membrane Proteins: Choosing the Appropriate Host

Florent Bernaudat, Annie Frelet-Barrand, [ ... ], Norbert Rolland

Total Protein Analysis as a Reliable Loading Control for Quantitative Fluorescent Western Blotting

Samantha L. Eaton, Sarah L. Roche, [ ... ], Thomas M. Wishart

A Comparative Study of Serum Exosome Isolation Using Differential Ultracentrifugation and Three Commercial Reagents

Inas Helwa, Jingwen Cai, [ ... ], Yutao Liu

AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach

Hannes M. Beyer, Patrick Gonschorek, [ ... ], Matias D. Zurbriggen

Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature

Chi-Yen Lin, Zhen Huang, [ ... ], Li Niu

A Simple and Rapid Method for Preparing a Cell-Free Bacterial Lysate for Protein Synthesis

Nitzan Krinsky, Maya Kaduri, [ ... ], Avi Schroeder

A Simplified Method for the Efficient Refolding and Purification of Recombinant Human GM-CSF

Christy A. Thomson, Melanie Olson, Linda M. Jackson, John W. Schrader

De Novo Designed Proteins from a Library of Artificial Sequences Function in Escherichia Coli and Enable Cell Growth

Michael A. Fisher, Kara L. McKinley, [ ... ], Michael H. Hecht

Improving the Expression of Recombinant Proteins in E. coli BL21 (DE3) under Acetate Stress: An Alkaline pH Shift Approach

Hengwei Wang, Fengqing Wang, [ ... ], Zixin Deng

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