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Polymerases

Putative endogenous filovirus VP35-like protein potentially functions as an IFN antagonist but not a polymerase cofactor

Tatsunari Kondoh, Rashid Manzoor, Naganori Nao, Junki Maruyama, Wakako Furuyama, Hiroko Miyamoto, Asako Shigeno, Makoto Kuroda, Keita Matsuno, Daisuke Fujikura, Masahiro Kajihara, Reiko Yoshida, Manabu Igarashi, Ayato Takada

published 17 Oct 2017

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Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.

Maggie R. Williams, Robert D. Stedtfeld, Cathrine Engle, Paul Salach, Umama Fakher, Tiffany Stedtfeld, Erin Dreelin, R. Jan Stevenson, Jo Latimore, Syed A. Hashsham

published 16 Oct 2017

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Biochemical characterization of recombinant influenza A polymerase heterotrimer complex: Polymerase activity and mechanisms of action of nucleotide analogs

Ona Barauskas, Weimei Xing, Esmeralda Aguayo, Madeleine Willkom, Annapurna Sapre, Michael Clarke, Gabriel Birkus, Brian E. Schultz, Roman Sakowicz, HyockJoo Kwon, Joy Y. Feng

published 11 Oct 2017

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Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol

Elizaveta O. Boldinova, Gorazd Stojkovič, Rafil Khairullin, Sjoerd Wanrooij, Alena V. Makarova

published 13 Sep 2017

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Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA

Konstantin A. Blagodatskikh, Vladimir M. Kramarov, Ekaterina V. Barsova, Alexey V. Garkovenko, Dmitriy S. Shcherbo, Andrew A. Shelenkov, Vera V. Ustinova, Maria R. Tokarenko, Simon C. Baker, Tatiana V. Kramarova, Konstantin B. Ignatov

published 11 Sep 2017

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Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans—Expression and characterization

Marcin Olszewski, Marta Śpibida, Maciej Bilek, Beata Krawczyk

published 01 Sep 2017

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RNA sequencing demonstrates large-scale temporal dysregulation of gene expression in stimulated macrophages derived from MHC-defined chicken haplotypes

Kristopher J. L. Irizarry, Eileen Downs, Randall Bryden, Jory Clark, Lisa Griggs, Renee Kopulos, Cynthia M. Boettger, Thomas J. Carr Jr., Calvin L. Keeler, Ellen Collisson, Yvonne Drechsler

published 28 Aug 2017

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In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR

Faqing Huang, Joseph Rankin Spangler, Allen Yang Huang

published 24 Aug 2017

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Variants of sequence family B Thermococcus kodakaraensis DNA polymerase with increased mismatch extension selectivity

Claudia Huber, Andreas Marx

published 23 Aug 2017

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Biochemical characterization of recombinant influenza A polymerase heterotrimer complex: Endonuclease activity and evaluation of inhibitors

Weimei Xing, Ona Barauskas, Thorsten Kirschberg, Anita Niedziela-Majka, Michael Clarke, Gabriel Birkus, Perry Weissburg, Xiaohong Liu, Brian E. Schultz, Roman Sakowicz, HyockJoo Kwon, Joy Y. Feng

published 15 Aug 2017

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Contribution of increased mutagenesis to the evolution of pollutants-degrading indigenous bacteria

Tanel Ilmjärv, Eve Naanuri, Maia Kivisaar

published 04 Aug 2017

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Molecular characterization of a novel cryptic virus infecting pigeonpea plants

Surender Kumar, Burra L. Subbarao, Reenu Kumari, Vipin Hallan

published 03 Aug 2017

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The emerging GII.P16-GII.4 Sydney 2012 norovirus lineage is circulating worldwide, arose by late-2014 and contains polymerase changes that may increase virus transmission

Christopher Ruis, Sunando Roy, Julianne R. Brown, David J. Allen, Richard A. Goldstein, Judith Breuer

published 29 Jun 2017

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