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Polymerases

Mutations in hepatitis B virus polymerase are associated with the postoperative survival of hepatocellular carcinoma patients

Fei Yin, Ying Xie, Haiyan Fan, Jingjing Zhang, Zhanjun Guo

published 29 Dec 2017

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Characterization of a not so new potexvirus from babaco (Vasconcellea x heilbornii)

Robert A. Alvarez-Quinto, Juan F. Cornejo-Franco, Diego F. Quito-Avila

published 15 Dec 2017

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Detection and molecular characterization of the novel recombinant norovirus GII.P16-GII.4 Sydney in southeastern Brazil in 2016

Débora Maria Pires Gonçalves Barreira, Túlio Machado Fumian, Marco André Loureiro Tonini, Lays Paula Bondi Volpini, Rodrigo Pratte Santos, Anézia Lima Chaves Ribeiro, José Paulo Gagliardi Leite, Márcia Terezinha Baroni de Moraes e Souza, Patrícia Brasil, Denise Cotrim da Cunha, Marize Pereira Miagostovich, Liliana Cruz Spano

published 13 Dec 2017

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Crystal structures of ternary complexes of archaeal B-family DNA polymerases

Heike M. Kropp, Karin Betz, Johannes Wirth, Kay Diederichs, Andreas Marx

published 06 Dec 2017

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TA-GC cloning: A new simple and versatile technique for the directional cloning of PCR products for recombinant protein expression

Athanasios Niarchos, Anastasia Siora, Evangelia Konstantinou, Vasiliki Kalampoki, George Lagoumintzis, Konstantinos Poulas

published 01 Nov 2017

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Putative endogenous filovirus VP35-like protein potentially functions as an IFN antagonist but not a polymerase cofactor

Tatsunari Kondoh, Rashid Manzoor, Naganori Nao, Junki Maruyama, Wakako Furuyama, Hiroko Miyamoto, Asako Shigeno, Makoto Kuroda, Keita Matsuno, Daisuke Fujikura, Masahiro Kajihara, Reiko Yoshida, Manabu Igarashi, Ayato Takada

published 17 Oct 2017

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Isothermal amplification of environmental DNA (eDNA) for direct field-based monitoring and laboratory confirmation of Dreissena sp.

Maggie R. Williams, Robert D. Stedtfeld, Cathrine Engle, Paul Salach, Umama Fakher, Tiffany Stedtfeld, Erin Dreelin, R. Jan Stevenson, Jo Latimore, Syed A. Hashsham

published 16 Oct 2017

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Biochemical characterization of recombinant influenza A polymerase heterotrimer complex: Polymerase activity and mechanisms of action of nucleotide analogs

Ona Barauskas, Weimei Xing, Esmeralda Aguayo, Madeleine Willkom, Annapurna Sapre, Michael Clarke, Gabriel Birkus, Brian E. Schultz, Roman Sakowicz, HyockJoo Kwon, Joy Y. Feng

published 11 Oct 2017

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Optimization of the expression, purification and polymerase activity reaction conditions of recombinant human PrimPol

Elizaveta O. Boldinova, Gorazd Stojkovič, Rafil Khairullin, Sjoerd Wanrooij, Alena V. Makarova

published 13 Sep 2017

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Improved DOP-PCR (iDOP-PCR): A robust and simple WGA method for efficient amplification of low copy number genomic DNA

Konstantin A. Blagodatskikh, Vladimir M. Kramarov, Ekaterina V. Barsova, Alexey V. Garkovenko, Dmitriy S. Shcherbo, Andrew A. Shelenkov, Vera V. Ustinova, Maria R. Tokarenko, Simon C. Baker, Tatiana V. Kramarova, Konstantin B. Ignatov

published 11 Sep 2017

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Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans—Expression and characterization

Marcin Olszewski, Marta Śpibida, Maciej Bilek, Beata Krawczyk

published 01 Sep 2017

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RNA sequencing demonstrates large-scale temporal dysregulation of gene expression in stimulated macrophages derived from MHC-defined chicken haplotypes

Kristopher J. L. Irizarry, Eileen Downs, Randall Bryden, Jory Clark, Lisa Griggs, Renee Kopulos, Cynthia M. Boettger, Thomas J. Carr Jr., Calvin L. Keeler, Ellen Collisson, Yvonne Drechsler

published 28 Aug 2017

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In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR

Faqing Huang, Joseph Rankin Spangler, Allen Yang Huang

published 24 Aug 2017

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