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Polymerases

Akaby—Cell-free protein expression system for linear templates

Wakana Sato, Judee Sharon, Christopher Deich, Nathaniel Gaut, Brock Cash, Aaron E. Engelhart, Katarzyna P. Adamala

published 07 Apr 2022

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Comparison of six methods for Loa loa genomic DNA extraction

Roland Dieki, Elsa-Rush Eyang-Assengone, Patrice Makouloutou-Nzassi, Félicien Bangueboussa, Edouard Nsi Emvo, Jean Paul Akue

published 21 Mar 2022

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Fluorinated oil-surfactant mixtures with the density of water: Artificial cells for synthetic biology

Roberto Laos, Steven Benner

published 20 Jan 2022

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Probing Loop-Mediated Isothermal Amplification (LAMP) targeting two gene-fragments of rose rosette virus

Andrea Salazar, Francisco M. Ochoa-Corona, Jennifer D. Olson, Binoy Babu, Mathews Paret

published 29 Nov 2021

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DNA bridges: A novel platform for single-molecule sequencing and other DNA-protein interaction applications

Maurizio Righini, Justin Costa, Wei Zhou

published 22 Nov 2021

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RNA polymerases in strict endosymbiont bacteria with extreme genome reduction show distinct erosions that might result in limited and differential promoter recognition

Cynthia Paola Rangel-Chávez, Edgardo Galán-Vásquez, Azucena Pescador-Tapia, Luis Delaye, Agustino Martínez-Antonio

published 29 Jul 2021

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Development of an efficient one-step real-time reverse transcription polymerase chain reaction method for severe acute respiratory syndrome-coronavirus-2 detection

Yukiko Nakura, Heng Ning Wu, Yuya Okamoto, Muneyuki Takeuchi, Koichiro Suzuki, Yoshitaka Tamura, Yuichiro Oba, Fumiko Nishiumi, Nobuaki Hatori, Shinsuke Fujiwara, Kiyoshi Yasukawa, Shinobu Ida, Itaru Yanagihara

published 04 Jun 2021

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Division of labor of Y-family polymerases in translesion-DNA synthesis for distinct types of DNA damage

Yuriko Inomata, Takuya Abe, Masataka Tsuda, Shunichi Takeda, Kouji Hirota

published 01 Jun 2021

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Producing molecular biology reagents without purification

Sanchita Bhadra, Vylan Nguyen, Jose-Angel Torres, Shaunak Kar, Stéphane Fadanka, Chiara Gandini, Harry Akligoh, Inyup Paik, Andre C. Maranhao, Jenny Molloy, Andrew D. Ellington

published 01 Jun 2021

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Overexpression of oncogenic H-Ras in hTERT-immortalized and SV40-transformed human cells targets replicative and specialized DNA polymerases for depletion

Wei-chung Tsao, Raquel Buj, Katherine M. Aird, Julia M. Sidorova, Kristin A. Eckert

published 07 May 2021

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Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection

Thomas G. W. Graham, Claire Dugast-Darzacq, Gina M. Dailey, Xammy Huu Wrynla, Erik Van Dis, Meagan N. Esbin, Abrar Abidi, Sarah A. Stanley, Xavier Darzacq, Robert Tjian

published 03 Feb 2021

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Detection of SARS-CoV-2 from raw patient samples by coupled high temperature reverse transcription and amplification

Johannes W. P. Kuiper, Timo Baade, Marcel Kremer, Ramon Kranaster, Linda Irmisch, Marcus Schuchmann, Johannes Zander, Andreas Marx, Christof R. Hauck

published 02 Nov 2020

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Interaction between influenza A virus nucleoprotein and PB2 cap-binding domain is mediated by RNA

Wun-Chung Szeto, Ho-Pan Hsia, Yun-Sang Tang, Pang-Chui Shaw

published 28 Sep 2020

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