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Polymerases

How to Open the Treasure Chest? Optimising DNA Extraction from Herbarium Specimens

Tiina Särkinen, Martijn Staats, [ ... ], Freek T. Bakker

Quick and Clean Cloning: A Ligation-Independent Cloning Strategy for Selective Cloning of Specific PCR Products from Non-Specific Mixes

Frank Thieme, Carola Engler, Romy Kandzia, Sylvestre Marillonnet

Improved PCR Performance Using Template DNA from Formalin-Fixed and Paraffin-Embedded Tissues by Overcoming PCR Inhibition

Dimo Dietrich, Barbara Uhl, [ ... ], Glen Kristiansen

Exponential Megapriming PCR (EMP) Cloning—Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints

Alexander Ulrich, Kasper R. Andersen, Thomas U. Schwartz

Optimizing Taq Polymerase Concentration for Improved Signal-to-Noise in the Broad Range Detection of Low Abundance Bacteria

Rudolph Spangler, Noel L. Goddard, David S. Thaler

PFunkel: Efficient, Expansive, User-Defined Mutagenesis

Elad Firnberg, Marc Ostermeier

Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection

Thomas G. W. Graham, Claire Dugast-Darzacq, [ ... ], Robert Tjian

Preparation of Phi29 DNA Polymerase Free of Amplifiable DNA Using Ethidium Monoazide, an Ultraviolet-Free Light-Emitting Diode Lamp and Trehalose

Hirokazu Takahashi, Hiroyuki Yamazaki, [ ... ], Toshiro Kobori

A Systematic Investigation of Parameters Influencing Droplet Rain in the Listeria monocytogenes prfA Assay - Reduction of Ambiguous Results in ddPCR

Anna Kristina Witte, Patrick Mester, [ ... ], Peter Rossmanith

A Complete Set of Nascent Transcription Rates for Yeast Genes

Vicent Pelechano, Sebastián Chávez, José E. Pérez-Ortín

Strand Invasion Based Amplification (SIBA®): A Novel Isothermal DNA Amplification Technology Demonstrating High Specificity and Sensitivity for a Single Molecule of Target Analyte

Mark J. Hoser, Hannu K. Mansukoski, Scott W. Morrical, Kevin E. Eboigbodin

A Mechanistic Model of PCR for Accurate Quantification of Quantitative PCR Data

Gregory J. Boggy, Peter J. Woolf

Evaluation of Inhibitor-Resistant Real-Time PCR Methods for Diagnostics in Clinical and Environmental Samples

Adrienne Trombley Hall, Ashley McKay Zovanyi, [ ... ], Timothy Devins Minogue

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