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Polymerases

An Efficient Multistrategy DNA Decontamination Procedure of PCR Reagents for Hypersensitive PCR Applications

Sophie Champlot, Camille Berthelot, [ ... ], Eva-Maria Geigl

DMSO and Betaine Greatly Improve Amplification of GC-Rich Constructs in De Novo Synthesis

Michael A. Jensen, Marilyn Fukushima, Ronald W. Davis

Design Parameters to Control Synthetic Gene Expression in Escherichia coli

Mark Welch, Sridhar Govindarajan, [ ... ], Claes Gustafsson

Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs

James A. Gagnon, Eivind Valen, [ ... ], Alexander F. Schier

Circular Polymerase Extension Cloning of Complex Gene Libraries and Pathways

Jiayuan Quan, Jingdong Tian

Examining Sources of Error in PCR by Single-Molecule Sequencing

Vladimir Potapov, Jennifer L. Ong

Emulsion PCR: A High Efficient Way of PCR Amplification of Random DNA Libraries in Aptamer Selection

Keke Shao, Weifeng Ding, [ ... ], Huimin Wang

The Behaviour of 5-Hydroxymethylcytosine in Bisulfite Sequencing

Yun Huang, William A. Pastor, [ ... ], Anjana Rao

A Practical Comparison of Ligation-Independent Cloning Techniques

Julian Stevenson, James R. Krycer, Lisa Phan, Andrew J. Brown

A Novel Universal Primer-Multiplex-PCR Method with Sequencing Gel Electrophoresis Analysis

Wentao Xu, Zhifang Zhai, [ ... ], Yunbo Luo

The Whole Set of Constitutive Promoters Recognized by RNA Polymerase RpoD Holoenzyme of Escherichia coli

Tomohiro Shimada, Yukiko Yamazaki, Kan Tanaka, Akira Ishihama

Benefits and Challenges with Applying Unique Molecular Identifiers in Next Generation Sequencing to Detect Low Frequency Mutations

Ruqin Kou, Ham Lam, [ ... ], Shihong Li

In vivo cloning of up to 16 kb plasmids in E. coli is as simple as PCR

Faqing Huang, Joseph Rankin Spangler, Allen Yang Huang

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