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Plasmid construction

Genome concentration, characterization, and integrity analysis of recombinant adeno-associated viral vectors using droplet digital PCR

Andrew Prantner, Dianna Maar

Characterization of a new IN-I-PpoI fusion protein and a homology-arm containing transgene cassette that improve transgene expression persistence and 28S rRNA gene-targeted insertion of lentiviral vectors

Alisa Nousiainen, Diana Schenkwein, Seppo Ylä-Herttuala

Ectodomain shedding of EGFR ligands serves as an activation readout for TRP channels

Manae Tatsumi, Takayuki Kishi, [ ... ], Asuka Inoue

A large-scale genetic screen identifies genes essential for motility in Agrobacterium fabrum

Diana G. Calvopina-Chavez, Robyn E. Howarth, [ ... ], Joel S. Griffitts

A novel SfaNI-like restriction-modification system in Caldicellulosiruptor extents the genetic engineering toolbox for this genus

Steve Swinnen, Christian Zurek, [ ... ], Albrecht Läufer

Efficient production of protein complexes in mammalian cells using a poxvirus vector

Robert Drillien, Karine Pradeau-Aubreton, [ ... ], Marc Ruff

Engineering a “detect and destroy” skin probiotic to combat methicillin-resistant Staphylococcus aureus

Changhui Guan, Peter J. Larson, [ ... ], Julia Oh

Using runaway replication to express polyhydroxyalkanoic acid (pha) genes from a novel marine bacterium in enteric bacteria: The influence of temperature and phasins on PHA accumulation

Dana Kolibachuk, Benjamin J. Ryder, [ ... ], Ashley Nieves

Cas9-mediated endogenous plasmid loss in Borrelia burgdorferi

Constantin N. Takacs, Yuko Nakajima, James E. Haber, Christine Jacobs-Wagner

A genetic approach to identify amino acids in Gcn1 required for Gcn2 activation

Susanne Gottfried, Siaosi M. B. M. J. Koloamatangi, [ ... ], Evelyn Sattlegger

The GGDEF-EAL protein CdgB from Azospirillum baldaniorum Sp245, is a dual function enzyme with potential polar localization

Víctor I. Viruega-Góngora, Iris S. Acatitla-Jácome, [ ... ], Alberto Ramírez-Mata

LupiQuant: A real-time PCR based assay for determining host-to-parasite DNA ratios of Onchocerca lupi and host Canis lupus from onchocercosis samples

Chandler C. Roe, Jennifer Urbanz, [ ... ], Jason W. Sahl

Versatile seamless DNA vector production in E. coli using enhanced phage lambda integrase

Suki Roy, Sabrina Peter, Peter Dröge

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