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Plasmid construction

Characterization of an accessory plasmid of Sinorhizobium meliloti and its two replication-modules

Abril Luchetti, Lucas G. Castellani, Andrés Martin Toscani, Antonio Lagares, María Florencia Del Papa, Gonzalo Torres Tejerizo, Mariano Pistorio

published 18 May 2023

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Tailoring baker’s yeast Saccharomyces cerevisiae for functional testing of channelrhodopsin

Sebastian Höler, Daniel Degreif, Florentine Stix, Shang Yang, Shiqiang Gao, Georg Nagel, Anna Moroni, Gerhard Thiel, Adam Bertl, Oliver Rauh

published 13 Apr 2023

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Adeno-Associated virus 8 delivers an immunomodulatory peptide to mouse liver more efficiently than to rat liver

Yuqing Wang, Ayrea Hurley, Marco De Giorgi, Mark R. Tanner, Rong-Chi Hu, Michael W. Pennington, William R. Lagor, Christine Beeton

published 11 Apr 2023

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Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene

Aya Bridgeland, Sudip Biswas, Nikolaos Tsakirpaloglou, Michael J. Thomson, Endang M. Septiningsih

published 05 Apr 2023

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IL-12p40 is essential but not sufficient for Francisella tularensis LVS clearance in chronically infected mice

Lara R. Mittereder, Jonathan Swoboda, Roberto De Pascalis, Karen L. Elkins

published 27 Mar 2023

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Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli

Riko Shirakawa, Kazuya Ishikawa, Kazuyuki Furuta, Chikara Kaito

published 24 Mar 2023

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Self-cleaving peptides for expression of multiple genes in Dictyostelium discoideum

Xinwen Zhu, Chiara Ricci-Tam, Emily R. Hager, Allyson E. Sgro

published 02 Mar 2023

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The DNA binding domain and the C-terminal region of DNA Ligase IV specify its role in V(D)J recombination

Vidyasagar Malashetty, Audrey Au, Jose Chavez, Mary Hanna, Jennifer Chu, Jesse Penna, Patricia Cortes

published 24 Feb 2023

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Research experimental design for the construction and identification of the pGEX-BCKD-E4A recombinant point-mutant plasmid

Tiannan Zhou, Huixian Wei, Jinjun Wang

published 24 Feb 2023

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pXOOY: A dual-function vector for expression of membrane proteins in Saccharomyces cerevisiae and Xenopus laevis oocytes

Victoria Amstrup Vold, Sebastian Glanville, Dan Arne Klaerke, Per Amstrup Pedersen

published 21 Feb 2023

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An optimized electrotransformation protocol for Lactobacillus jensenii

Elsa Fristot, Thomas Bessede, Miguel Camacho Rufino, Pauline Mayonove, Hung-Ju Chang, Ana Zuniga, Anne-Laure Michon, Sylvain Godreuil, Jérôme Bonnet, Guillaume Cambray

published 17 Feb 2023

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In vitro assembly of plasmid DNA for direct cloning in Lactiplantibacillus plantarum WCSF1

Marc Blanch-Asensio, Sourik Dey, Shrikrishnan Sankaran

published 16 Feb 2023

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TFIID dependency of steady-state mRNA transcription altered epigenetically by simultaneous functional loss of Taf1 and Spt3 is Hsp104-dependent

Ryo Iwami, Naoki Takai, Minenosuke Matsutani, Yuh Shiwa, Haruki Kokubo, Koji Kasahara, Tetsuro Kokubo

published 09 Feb 2023

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