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Plasmid construction

GreenGate 2.0: Backwards compatible addons for assembly of complex transcriptional units and their stacking with GreenGate

Marcel Piepers, Katarina Erbstein, Jazmin Reyes-Hernandez, Changzheng Song, Tomas Tessi, Vesta Petrasiunaite, Naja Faerber, Kathrin Distel, Alexis Maizel

published 08 Sep 2023

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Co-expression of nitrogenase proteins in cotton (Gossypium hirsutum L.)

Yimin Shang, Wenfang Guo, Xiaomeng Liu, Lei Ma, Dehu Liu, Sanfeng Chen

published 24 Aug 2023

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The ubiquitin-like modifier FAT10 covalently modifies HUWE1 and strengthens the interaction of AMBRA1 and HUWE1

Stefanie Mueller, Johanna Bialas, Stella Ryu, Nicola Catone, Annette Aichem

published 14 Aug 2023

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Two opposing gene expression patterns within ATRX aberrant neuroblastoma

Michael R. van Gerven, Linda Schild, Jennemiek van Arkel, Bianca Koopmans, Luuk A. Broeils, Loes A. M. Meijs, Romy van Oosterhout, Max M. van Noesel, Jan Koster, Sander R. van Hooff, Jan J. Molenaar, Marlinde L. van den Boogaard

published 04 Aug 2023

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SUNi mutagenesis: Scalable and uniform nicking for efficient generation of variant libraries

Taylor L. Mighell, Ignasi Toledano, Ben Lehner

published 07 Jul 2023

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Peptide encoded by lncRNA BVES-AS1 promotes cell viability, migration, and invasion in colorectal cancer cells via the SRC/mTOR signaling pathway

Weiwei Zheng, Yingchang Guo, Guangtan Zhang, Junwei Bai, Yucheng Song, Xiaofei Song, Qinhui Zhu, Xuebin Bao, Gang Wu, Chao Zhang

published 22 Jun 2023

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A simplified function-first method for the discovery and optimization of bispecific immune engaging antibodies

Alex Shepherd, Bigitha Bennychen, Anne Marcil, Darin Bloemberg, Robert A. Pon, Risini D. Weeratna, Scott McComb

published 22 Jun 2023

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Development of a rapid and sensitive real-time diagnostic assay to detect and quantify Aphanomyces invadans, the causative agent of epizootic ulcerative syndrome

Diem Tho Ho, Nameun Kim, Yoonhang Lee, Dongbin Yun, MinJi Sung, El-Matbouli Mansour, P. K. Pradhan, Neeraj Sood, Wi-Sik Kim, Chan-Il Park, Ki Hong Kim, Do-Hyung Kim

published 15 Jun 2023

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A short peptide LINC00665_18aa encoded by lncRNA LINC00665 suppresses the proliferation and migration of osteosarcoma cells through the regulation of the CREB1/RPS6KA3 interaction

Junwei Pan, Ming Liu, Xiaozong Duan, Dan Wang

published 07 Jun 2023

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Characterization of an accessory plasmid of Sinorhizobium meliloti and its two replication-modules

Abril Luchetti, Lucas G. Castellani, Andrés Martin Toscani, Antonio Lagares, María Florencia Del Papa, Gonzalo Torres Tejerizo, Mariano Pistorio

published 18 May 2023

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Tailoring baker’s yeast Saccharomyces cerevisiae for functional testing of channelrhodopsin

Sebastian Höler, Daniel Degreif, Florentine Stix, Shang Yang, Shiqiang Gao, Georg Nagel, Anna Moroni, Gerhard Thiel, Adam Bertl, Oliver Rauh

published 13 Apr 2023

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Adeno-Associated virus 8 delivers an immunomodulatory peptide to mouse liver more efficiently than to rat liver

Yuqing Wang, Ayrea Hurley, Marco De Giorgi, Mark R. Tanner, Rong-Chi Hu, Michael W. Pennington, William R. Lagor, Christine Beeton

published 11 Apr 2023

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Optimization of gene editing in cowpea through protoplast transformation and agroinfiltration by targeting the phytoene desaturase gene

Aya Bridgeland, Sudip Biswas, Nikolaos Tsakirpaloglou, Michael J. Thomson, Endang M. Septiningsih

published 05 Apr 2023

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