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Plasmid construction

A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli

Chuan-Wei Jang, Terry Magnuson

Screening, Expression, Purification and Functional Characterization of Novel Antimicrobial Peptide Genes from Hermetia illucens (L.)

Osama Elhag, Dingzhong Zhou, [ ... ], Jibin Zhang

Expression and Purification of Recombinant Hemoglobin in Escherichia coli

Chandrasekhar Natarajan, Xiaoben Jiang, [ ... ], Jay F. Storz

An Engineered Yeast Efficiently Secreting Penicillin

Loknath Gidijala, Jan A. K. W. Kiel, [ ... ], Ida J. van der Klei

Boundaries of the Origin of Replication: Creation of a pET-28a-Derived Vector with p15A Copy Control Allowing Compatible Coexistence with pET Vectors

Sarmitha Sathiamoorthy, Jumi A. Shin

High-Level Fluorescence Labeling of Gram-Positive Pathogens

Simone Aymanns, Stefanie Mauerer, [ ... ], Barbara Spellerberg

Transcriptional Signature and Memory Retention of Human-Induced Pluripotent Stem Cells

Maria C. N. Marchetto, Gene W. Yeo, [ ... ], Alysson R. Muotri

MosSCI and Gateway Compatible Plasmid Toolkit for Constitutive and Inducible Expression of Transgenes in the C. elegans Germline

Eva Zeiser, Christian Frøkjær-Jensen, Erik Jorgensen, Julie Ahringer

Tagging the Expressed Protein with 6 Histidines: Rapid Cloning of an Amplicon with Three Options

Manika Indrajit Singh, Vikas Jain

Expanding the Repertoire of Gene Tools for Precise Manipulation of the Clostridium difficile Genome: Allelic Exchange Using pyrE Alleles

Yen Kuan Ng, Muhammad Ehsaan, [ ... ], Nigel P. Minton

Standard Addition Quantitative Real-Time PCR (SAQPCR): A Novel Approach for Determination of Transgene Copy Number Avoiding PCR Efficiency Estimation

Yuji Huang, Xueren Yin, [ ... ], Kunsong Chen

Induced Pluripotent Stem Cells Generated from Human Adipose-Derived Stem Cells Using a Non-Viral Polycistronic Plasmid in Feeder-Free Conditions

Xinjian Qu, Tianqing Liu, [ ... ], Dan Ge

In Vitro Assembly of Multiple DNA Fragments Using Successive Hybridization

Xinglin Jiang, Jianming Yang, [ ... ], Mo Xian

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