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Plasmid construction

Cloning of maize chitinase 1 gene and its expression in genetically transformed rice to confer resistance against rice blast caused by Pyricularia oryzae

Sadaf Anwaar, Nyla Jabeen, Khawaja Shafique Ahmad, Saima Shafique, Samra Irum, Hammad Ismail, Siffat Ullah Khan, Ateeq Tahir, Nasir Mehmood, Mark L. Gleason

published 16 Jan 2024

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Molecular cloning and functional analysis of 4-coumarate: CoA ligases from Marchantia paleacea and their roles in lignin and flavanone biosynthesis

Shuai Gao, Xin-Yan Liu, Rong Ni, Jie Fu, Hui Tan, Ai-Xia Cheng, Hong-Xiang Lou

published 08 Jan 2024

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A novel method for quantitation of AAV genome integrity using duplex digital PCR

Lauren Tereshko, Xiaohui Zhao, Jake Gagnon, Tinchi Lin, Trevor Ewald, Yu Wang, Marina Feschenko, Cullen Mason

published 14 Dec 2023

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Cas9 is mostly orthogonal to human systems of DNA break sensing and repair

Ekaterina A. Maltseva, Inna A. Vasil’eva, Nina A. Moor, Daria V. Kim, Nadezhda S. Dyrkheeva, Mikhail M. Kutuzov, Ivan P. Vokhtantsev, Lilya M. Kulishova, Dmitry O. Zharkov, Olga I. Lavrik

published 29 Nov 2023

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Assessment of oligomerization of bacterial micro-compartment shell components with the tripartite GFP reporter technology

Lucie Barthe, Vanessa Soldan, Luis F. Garcia-Alles

published 27 Nov 2023

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Sensing chemical-induced genotoxicity and oxidative stress via yeast-based reporter assays using NanoLuc luciferase

Minami Shichinohe, Shun Ohkawa, Yuu Hirose, Toshihiko Eki

published 22 Nov 2023

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FlgV forms a flagellar motor ring that is required for optimal motility of Helicobacter pylori

Jack M. Botting, Shoichi Tachiyama, Katherine H. Gibson, Jun Liu, Vincent J. Starai, Timothy R. Hoover

published 17 Nov 2023

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Generation of porcine PK-15 cells lacking the Ifnar1 or Stat2 gene to optimize the efficiency of viral isolation

Maya Shofa, Akatsuki Saito

published 08 Nov 2023

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Human BST2 inhibits rabies virus release independently of cysteine-linked dimerization and asparagine-linked glycosylation

Nathiphat Tanwattana, Nanchaya Wanasen, Yuparat Jantraphakorn, Kanjana Srisutthisamphan, Thanathom Chailungkarn, Suwimon Boonrungsiman, Boonlert Lumlertdacha, Porntippa Lekchareonsuk, Challika Kaewborisuth

published 03 Nov 2023

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A simple and efficient protocol for generating transgenic hairy roots using Agrobacterium rhizogenes

Shaun Ferguson, Nikolaj B. Abel, Dugald Reid, Lene H. Madsen, Thi-Bich Luu, Kasper R. Andersen, Jens Stougaard, Simona Radutoiu

published 01 Nov 2023

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Identification of the functional domain of the dense core vesicle biogenesis factor HID-1

Blake H. Hummer, Theodore Carter, Breanna L. Sellers, Jenna D. Triplett, Cedric S. Asensio

published 26 Sep 2023

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Development of a novel in vitro model to study the modulatory role of the respiratory complex I in macrophage effector functions

Pablo Serrano-Lorenzo, Dino Gobelli, Rocío Garrido-Moraga, María J. Esteban-Amo, José R. López-López, Antonio Orduña, Miguel A. de la Fuente, Miguel A. Martín, María Simarro

published 19 Sep 2023

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Engineering conjugative CRISPR-Cas9 systems for the targeted control of enteric pathogens and antibiotic resistance

Haiqing Sheng, Sarah Wu, Yansong Xue, Wei Zhao, Allan B. Caplan, Carolyn J. Hovde, Scott A. Minnich

published 12 Sep 2023

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