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Plasmid construction

In Vivo Structure of the E. coli FtsZ-ring Revealed by Photoactivated Localization Microscopy (PALM)

Guo Fu, Tao Huang, [ ... ], Jie Xiao

Sensitive Detection of Gene Expression in Mycobacteria under Replicating and Non-Replicating Conditions Using Optimized Far-Red Reporters

Paul Carroll, Lise J. Schreuder, [ ... ], Tanya Parish

Efficient Conversion of Astrocytes to Functional Midbrain Dopaminergic Neurons Using a Single Polycistronic Vector

Russell C. Addis, Fu-Chun Hsu, [ ... ], John D. Gearhart

A Modified RMCE-Compatible Rosa26 Locus for the Expression of Transgenes from Exogenous Promoters

Jan S. Tchorz, Thomas Suply, [ ... ], Matthias Mueller

Large-Scale Protein-Protein Interaction Analysis in Arabidopsis Mesophyll Protoplasts by Split Firefly Luciferase Complementation

Jian-Feng Li, Jenifer Bush, [ ... ], Matthew McCormack

Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost

Roger M. Benoit, Christian Ostermeier, [ ... ], Manfred Auer

A Super-Ecliptic, pHluorin-mKate2, Tandem Fluorescent Protein-Tagged Human LC3 for the Monitoring of Mammalian Autophagy

Isei Tanida, Takashi Ueno, Yasuo Uchiyama

Highly multiplexed genome engineering using CRISPR/Cas9 gRNA arrays

Morito Kurata, Natalie K. Wolf, [ ... ], Branden S. Moriarity

Efficient genome editing using tRNA promoter-driven CRISPR/Cas9 gRNA in Aspergillus niger

Letian Song, Jean-Paul Ouedraogo, [ ... ], Adrian Tsang

Quantitative Co-Expression of Proteins at the Single Cell Level – Application to a Multimeric FRET Sensor

Joachim Goedhart, Laura van Weeren, [ ... ], Theodorus W.J. Gadella Jr

Protein C-Terminal Labeling and Biotinylation Using Synthetic Peptide and Split-Intein

Gerrit Volkmann, Xiang-Qin Liu

Production and Characterization of Novel Recombinant Adeno-Associated Virus Replicative-Form Genomes: A Eukaryotic Source of DNA for Gene Transfer

Lina Li, Emilios K. Dimitriadis, [ ... ], Robert M. Kotin

Fluorescent Protein-Based Methods for On-Plate Screening of Gene Insertion

Stanley S. C. Wong, Kevin Truong

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