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Plasmid construction

Enhancing Production of Bio-Isoprene Using Hybrid MVA Pathway and Isoprene Synthase in E. coli

Jianming Yang, Mo Xian, [ ... ], Wei Liu

Rational Diversification of a Promoter Providing Fine-Tuned Expression and Orthogonal Regulation for Synthetic Biology

Benjamin A. Blount, Tim Weenink, Serge Vasylechko, Tom Ellis

Monitoring of Gene Expression in Bacteria during Infections Using an Adaptable Set of Bioluminescent, Fluorescent and Colorigenic Fusion Vectors

Frank Uliczka, Fabio Pisano, [ ... ], Petra Dersch

System for Stable β-Estradiol-Inducible Gene Expression in the Moss Physcomitrella patens

Minoru Kubo, Akihiro Imai, [ ... ], Mitsuyasu Hasebe

Isolation of Genes Involved in Biofilm Formation of a Klebsiella pneumoniae Strain Causing Pyogenic Liver Abscess

Meng-Chuan Wu, Tzu-Lung Lin, [ ... ], Jin-Town Wang

Efficient Genome Engineering of Toxoplasma gondii Using CRISPR/Cas9

Saima M. Sidik, Caroline G. Hackett, [ ... ], Sebastian Lourido

Optimized Expression of Full-Length IgG1 Antibody in a Common E. coli Strain

Conrad En Zuo Chan, Angeline Pei Chiew Lim, [ ... ], Brendon John Hanson

Quantification of Plasmid Copy Number with Single Colour Droplet Digital PCR

Magdalena Plotka, Mateusz Wozniak, Tadeusz Kaczorowski

Plasmid interference for curing antibiotic resistance plasmids in vivo

Muhammad Kamruzzaman, Shereen Shoma, [ ... ], Jonathan R. Iredell

A Rapid and Simple Method for DNA Engineering Using Cycled Ligation Assembly

Theodore L. Roth, Ljiljana Milenkovic, Matthew P. Scott

Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly

Andreas I. Andreou, Naomi Nakayama

Selection of Single Domain Antibodies from Immune Libraries Displayed on the Surface of E. coli Cells with Two β-Domains of Opposite Topologies

Valencio Salema, Elvira Marín, [ ... ], Luis Ángel Fernández

Ethylene Synthesis and Regulated Expression of Recombinant Protein in Synechocystis sp. PCC 6803

Fernando Guerrero, Verónica Carbonell, [ ... ], Patrik R. Jones

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